Notch and Hedgehog in the thymus/parathyroid common primordium: Crosstalk in organ formation
| Autor: | Joana Clara Silva, Hélia Neves, Marta Figueiredo, Vitor Proa, António Cidadão, Isabel Alcobia, Rita Zilhão, Ana Sofia Santos |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty animal structures Pharyngeal pouch Organogenesis Notch signaling pathway Chick Embryo Coturnix Thymus Gland Biology Avian Proteins Parathyroid Glands 03 medical and health sciences stomatognathic system Internal medicine medicine Animals Hedgehog Proteins Primordium Molecular Biology Hedgehog In Situ Hybridization Receptors Notch GATA3 Gene Expression Regulation Developmental FOXN1 Receptor Cross-Talk Cell Biology Immunohistochemistry Cell biology Crosstalk (biology) 030104 developmental biology medicine.anatomical_structure Endocrinology embryonic structures Endoderm Signal Transduction Developmental Biology |
| Zdroj: | Developmental Biology. 418:268-282 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2016.08.012 |
| Popis: | The avian thymus and parathyroids (T/PT) common primordium derives from the endoderm of the third and fourth pharyngeal pouches (3/4PP). The molecular mechanisms that govern T/PT development are not fully understood. Here we study the effects of Notch and Hedgehog (Hh) signalling modulation during common primordium development using in vitro, in vivo and in ovo approaches. The impairment of Notch activity reduced Foxn1/thymus-fated and Gcm2/Pth/parathyroid-fated domains in the 3/4PP and further compromised the development of the parathyroid glands. When Hh signalling was abolished, we observed a reduction in the Gata3/Gcm2- and Lfng-expression domains at the median/anterior and median/posterior territories of the pouches, respectively. In contrast, the Foxn1 expression-domain at the dorsal tip of the pouches expanded ventrally into the Lfng-expression domain. This study offers novel evidence on the role of Notch signalling in T/PT common primordium development, in an Hh-dependent manner. |
| Databáze: | OpenAIRE |
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