Evaluation of different conditions and culture media for the recovery ofAeromonasspp. from water and shellfish samples
| Autor: | Maria José Figueras, Fadua Latif-Eugenín, Roxana Beaz-Hidalgo |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Bacteriological Techniques Veterinary medicine biology Fresh Water General Medicine Isolation (microbiology) biology.organism_classification Applied Microbiology and Biotechnology Culture Media Aeromonas species Microbiology 03 medical and health sciences 030104 developmental biology Aeromonas Direct plating Prevalence studies Aeromonas media Shellfish Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 121:883-891 |
| ISSN: | 1364-5072 |
| Popis: | To perform a comparative study for determining the optimum culture method (direct plating or enrichment) and medium (ampicillin dextrin agar (ADA), starch ampicillin agar (SAA), bile salts irgasan brilliant green modified (BIBG-m)) for recovering Aeromonas species from water and shellfish samples.By direct culture, Aeromonas was detected in 65% (13/20) of the water samples and in 54·5% (6/11) of the shellfish samples. However, when a pre-enrichment step was included, the number of positive water samples increased to 75% (15/20) and the ones of shellfish to 90·1% (10/11). The enriched culture significantly favoured (P 0·05) the isolation of Aeromonas allosaccharophila from water, Aeromonas salmonicida from shellfish and Aeromonas caviae from both types of samples. The most specific (P 0·05) culture medium for detecting Aeromonas from water was ADA. However, no differences were observed in the case of shellfish samples (P 0·05). Isolation of Aeromonas media from water was favoured (P 0·05) in the ADA medium, while SAA enhanced (P 0·05) the isolation of Aer. salmonicida from shellfish.The culture method and medium used influenced the recovery of some Aeromonas species from water and shellfish samples.This fact should be considered in future prevalence studies to avoid overestimating the above mentioned Aeromonas species. |
| Databáze: | OpenAIRE |
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