Gastroprotective effects and antimicrobial activity of Lithraea molleoides and isolated compounds against Helicobacter pylori
| Autor: | José Roberto Cury Saad, Angel Gabriel Salinas Ibáñez, A. O. M. María, Lilian Eugenia Pelzer, Maria Filomena Garro, Andrea Celeste Arismendi Sosa, A.E. Vega |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Biología Anacardiaceae Phytochemicals Microbial Sensitivity Tests Pharmacology Ulcer index Protective Agents Ferulic acid Ciencias Biológicas Rutin chemistry.chemical_compound Mice Drug Discovery Caffeic acid Toxicity Tests Acute Animals Gallic acid Stomach Ulcer Rats Wistar Lithraea molleoides biology Ethanol Helicobacter pylori Plant Extracts Gastric ulcer Antimicrobial biology.organism_classification Acute toxicity Anti-Bacterial Agents chemistry Female Gastroprotection CIENCIAS NATURALES Y EXACTAS Phytotherapy |
| Zdroj: | Journal of ethnopharmacology. 176 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance Lithraea molleoides (Vell.) Engl. (Anacardiaceae) is a medicinal plant traditionally used in South America to treat various ailments, including diseases of the digestive system. Aim of the study To evaluate the in vivo antiulcer and antimicrobial activities against Helicobacter pylori of L. molleoides and its isolated compounds. Materials and methods Methanolic extract 250 and 500 mg/kg, (LmE 250 and LmE 500, respectively) and infusions, 10 g and 20 g en 100 mL (LmI 10 and LmI 20, respectively) of L. molleoides was evaluated for antiulcer activity against 0.6N HCl, 0.2N NaOH, 200 mg/kg acetilsalicilic acid and absolute ethanol-induced gastric ulcers in rats. The degree of erosion in the glandular part of the stomach was assessed from a scoring system. Acute toxicity in mice was also evaluated. The antiulcer effect of the isolated compounds (catechol, mannitol, rutin, gallic acid, ferulic acid and caffeic acid, 100 mg/kg) was evaluated against absolute ethanol-induced gastric ulcers in rats. The anti-Helicobacter pylori activity of L. molleoides and isolated compounds was performed using broth dilution methods. Results The LmE 250, LmE 500, LmI 10 and LmI 20 produced significant inhibition on the ulcer index in 0.6N HCl, 0.2N NaOH, 200 mg/kg acetilsalicilic acid and absolute ethanol- induced gastric ulcers in rats. The isolated compounds, catechol, mannitol, rutin, ferulic acid and caffeic acid were active in absolute ethanol- induced gastric ulcers in rats. L. molleoides and different compounds showed antimicrobial activity in all strains tested. The lowest MIC value (0. 5 μg/mL) was obtained with catechol in six of eleven strains assayed. No signs of toxicity were observed with doses up to 2 g/kg in an acute toxicity assay. Conclusion These findings indicate that L. molleoides displays potential antiulcerogenic and antimicrobial activities and the identification of active principles could support the use of this plant for the treatment of digestive affections. Fil: Garro, Maria Filomena. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Salinas Ibáñez, Angel Gabriel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Vega, Alba Edith. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Arismendi Sosa, Andrea Celeste. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pelzer, Lilian. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Saad, José Roberto. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Investigaciones en Tecnología Química; Argentina Fil: Maria, Alejandra Olivia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina |
| Databáze: | OpenAIRE |
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