Characterization of binding by repaglinide and nateglinide with glycated human serum albumin using high‐performance affinity microcolumns

Autor:	Susan T. Ovbude, Pingyang Tao, Zhao Li, David S. Hage
Rok vydání:	2022
Předmět:	Filtration and Separation Analytical Chemistry
Zdroj:	Journal of Separation Science. 45:4176-4186
ISSN:	1615-9314 1615-9306
Popis:	High-performance affinity microcolumns were used to characterize binding by the anti-diabetic drugs repaglinide and nateglinide with normal and glycated forms of human serum albumin. The microcolumns contained only nmol amounts of protein and provided a detailed analysis of these drug interactions with good precision and in a matter of minutes per experiment. The overall binding by repaglinide to normal and glycated albumin fits a model with two types of binding sites: a set of one or two moderate-to-high affinity regions and a larger set of weaker regions with association equilibrium constants of ∼10
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf16e158fbe61bd86b71b5b39fe01a75 https://doi.org/10.1002/jssc.202200686 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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