Characterization of binding by repaglinide and nateglinide with glycated human serum albumin using high‐performance affinity microcolumns
Autor: | Susan T. Ovbude, Pingyang Tao, Zhao Li, David S. Hage |
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Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Journal of Separation Science. 45:4176-4186 |
ISSN: | 1615-9314 1615-9306 |
Popis: | High-performance affinity microcolumns were used to characterize binding by the anti-diabetic drugs repaglinide and nateglinide with normal and glycated forms of human serum albumin. The microcolumns contained only nmol amounts of protein and provided a detailed analysis of these drug interactions with good precision and in a matter of minutes per experiment. The overall binding by repaglinide to normal and glycated albumin fits a model with two types of binding sites: a set of one or two moderate-to-high affinity regions and a larger set of weaker regions with association equilibrium constants of ∼10 |
Databáze: | OpenAIRE |
Externí odkaz: | |
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