Endothelial progenitor cell secretome delivered by novel polymeric nanoparticles in ischemic hindlimb
| Autor: | Maria Chiara Barsotti, Nadia Ucciferri, Tatiana Santoni, Silvia Rocchiccioli, Anna Maria Piras, Rossella Di Stefano, Angela Pucci, Francesca Felice, Antonella Cecchettini, Silvia Burchielli, Roberto Solaro, Angelina Altomare, Federica Chiellini |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Proteomics 0301 basic medicine Cell Survival Polymers Angiogenesis Ischemia Neovascularization Physiologic Pharmaceutical Science Hindlimb Endothelial progenitor cell Conditioned medium Endothelial progenitor cells Hindlimb ischemia Hypoxia Nanoparticles 3003 Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine In vivo Human Umbilical Vein Endothelial Cells medicine Animals Humans Progenitor cell Cells Cultured Endothelial Progenitor Cells Matrigel Chemistry Hypoxia (medical) medicine.disease Cell biology 030104 developmental biology embryonic structures cardiovascular system medicine.symptom 030217 neurology & neurosurgery circulatory and respiratory physiology |
| Zdroj: | International Journal of Pharmaceutics. 542:82-89 |
| ISSN: | 0378-5173 |
| DOI: | 10.1016/j.ijpharm.2018.03.015 |
| Popis: | Endothelial progenitor cells (EPCs) contribute to ischemic tissue repair by paracrine secretion up-regulated by hypoxia. In this study we use novel nanoparticles (NPs) as carriers for a controlled release of EPC secretome (CM) to improve their angiogenic properties. The in vivo effect in ischemic hindlimb rat model was evaluated, comparing hypoxic EPC-CM-NPs with hypoxic EPC-CM alone. A proteomic characterization of hypoxic CM and the in vitro effect on endothelial cells (HUVECs) were also performed. Up to 647 protein, 17 of which with angiogenic properties, were upregulated by hypoxia. Moreover, hypoxic EPC-CM significantly promoted capillary-like structures on Matrigel. A significant increase of blood perfusion in ischemic limbs at 2 weeks with EPC-CM-loaded NPs as compared to both EPC-CM and control and a significant increase of capillary formation were observed. The use of EPC-CM-NPs significantly improved neoangiogenesis in vivo, underlining the advantages of controlled release in regenerative medicine. |
| Databáze: | OpenAIRE |
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