Early detection of osteoarthritis in the rat with an antibody specific to type II collagen modified by reactive oxygen species
| Autor: | Christian Brenneis, Donata Harazin, Didier Merciris, Anne Gigout, Sven Lindemann, Ahuva Nissim, Louise M. Topping |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases Cartilage Articular Pathology medicine.medical_specialty Anterior cruciate ligament Type II collagen Chondrocyte hypertrophy Diseases of the musculoskeletal system Osteoarthritis 03 medical and health sciences 0302 clinical medicine Chondrocytes Medicine Animals 030203 arthritis & rheumatology Territorial matrix Collagen type X business.industry Cartilage Hypertrophy medicine.disease Staining Rats Disease Models Animal 030104 developmental biology medicine.anatomical_structure RC925-935 Collagen type II business Reactive Oxygen Species Medial meniscus Research Article |
| Zdroj: | Arthritis Research & Therapy Arthritis Research & Therapy, Vol 23, Iss 1, Pp 1-11 (2021) |
| ISSN: | 1478-6362 1478-6354 |
| Popis: | Background Osteoarthritis (OA) is a disease of the whole joint, with articular cartilage breakdown as a major characteristic. Inflammatory mediators, proteases, and oxidants produced by chondrocytes are known to be responsible for driving cartilage degradation. Nevertheless, the early pathogenic events are still unclear. To investigate this, we employed an antibody that is specific to oxidative post-translationally modified collagen type II (anti-oxPTM-CII) to detect early cartilage pathogenic changes in two rat models of OA. Methods The animals underwent surgery for destabilization of the medial meniscus (DMM) and were sacrificed after 3, 5, 7, 14, and 28 days. Alternatively, anterior cruciate ligament transection with partial meniscectomy (ACLT+pMx) was performed and animals were sacrificed after 1, 3, 5, 7, and 14 days. Joints were stained with toluidine blue and saffron du Gatinais for histological scoring, anti-oxPTM-CII, and anti-collagen type X antibodies (anti-CX). Results We observed positive oxPTM-CII staining as early as 1 or 3 days after ACLT+pMx or DMM surgeries, respectively, before overt cartilage lesions were visible. oxPTM-CII was located mostly in the deep zone of the medial tibial cartilage, in the pericellular and territorial matrix of hypertrophic chondrocytes, and co-localized with CX staining. Staining was weak or absent for the lateral compartment or the contralateral knees except at later time points. Conclusion The results demonstrate that oxidant production and chondrocyte hypertrophy occur very early in the onset of OA, possibly initiating the pathogenic events of OA. We propose to use anti-oxPTM-CII as an early biomarker for OA ahead of radiographic changes. |
| Databáze: | OpenAIRE |
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