Nuclear magnetic resonance relaxation studies of poly(hydroxybutyrate) in whole cells and in artificial granules
| Autor: | James Keeler, Jeremy K. M. Sanders, Daniel M. Horowitz, Graeme L. Shaw, Melissa K. Melby |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Magnetic Resonance Spectroscopy
Chemistry Polyesters Relaxation (NMR) technology industry and agriculture Hydroxybutyrates macromolecular substances General Medicine Alcaligenes eutrophus Biochemistry Freeze Drying Nuclear magnetic resonance Structural Biology Transverse relaxation Static field lipids (amino acids peptides and proteins) Alcaligenes Spectroscopy Molecular Biology |
| Zdroj: | International Journal of Biological Macromolecules. 16:59-63 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/0141-8130(94)90015-9 |
| Popis: | The physical state of poly(hydroxybutyrate) (PHB) in whole cells and in the form of artificial biomimetic granules has been probed using 13C nuclear magnetic resonance (NMR) spectroscopy. Studies on varying concentrations of whole cells of Alcaligenes eutrophus show that changes in the line widths of PHB in whole cells do not correlate with changes in transverse relaxation times. Solid-state magic-angle spinning NMR studies demonstrate that the line broadening results from a reduction in the static field homogeneity rather than from intrinsic properties of the PHB within the cells. Transverse and longitudinal relaxation times of PHB in whole cells and in artificial granules are similar, indicating similarities in structure and mobility. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|