Role of Trehalose in Salinity and Temperature Tolerance in the Model Halophilic Bacterium Chromohalobacter salexigens
| Autor: | Laszlo N. Csonka, Mercedes Reina-Bueno, Montserrat Argandoña, Javier Rodríguez-Moya, Fernando Iglesias-Guerra, Joaquín J. Nieto, Carmen Vargas, Manuel Salvador |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Microbiología y Parasitología, Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Salinity
Hot Temperature Magnetic Resonance Spectroscopy Osmotic shock lcsh:Medicine Ectoine Biochemistry Microbiology Molecular Genetics Desiccation tolerance chemistry.chemical_compound Escherichia coli Genetics Chromohalobacter Carbon Radioisotopes Desiccation lcsh:Science Biology Cells Cultured Multidisciplinary biology lcsh:R Osmolar Concentration Trehalose Computational Biology Bacteriology biology.organism_classification Halophile Metabolism chemistry Glucosyltransferases Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Mutation Halotolerance lcsh:Q Research Article Biotechnology |
| Zdroj: | Department of Biological Sciences Faculty Publications PLoS ONE PLoS ONE, Vol 7, Iss 3, p e33587 (2012) idUS. Depósito de Investigación de la Universidad de Sevilla instname |
| Popis: | The disaccharide trehalose is considered as a universal stress molecule, protecting cells and biomolecules from injuries imposed by high osmolarity, heat, oxidation, desiccation and freezing. Chromohalobacter salexigens is a halophilic and extremely halotolerant γ-proteobacterium of the family Halomonadaceae. In this work, we have investigated the role of trehalose as a protectant against salinity, temperature and desiccation in C. salexigens. A mutant deficient in the trehalose-6-phosphate synthase gene (otsA::Ω) was not affected in its salt or heat tolerance, but double mutants ectoine- and trehalose-deficient, or hydroxyectoine-reduced and trehalose-deficient, displayed an osmo- and thermosensitive phenotype, respectively. This suggests a role of trehalose as a secondary solute involved in osmo- (at least at low salinity) and thermoprotection of C. salexigens. Interestingly, trehalose synthesis was osmoregulated at the transcriptional level, and thermoregulated at the post-transcriptional level, suggesting that C. salexigens cells need to be pre-conditioned by osmotic stress, in order to be able to quickly synthesize trehalose in response to heat stress. C. salexigens was more sensitive to desiccation than E. coli and desiccation tolerance was slightly improved when cells were grown at high temperature. Under these conditions, single mutants affected in the synthesis of trehalose or hydroxyectoine were more sensitive to desiccation than the wild-type strain. However, given the low survival rates of the wild type, the involvement of trehalose and hydroxyectoine in C. salexigens response to desiccation could not be firmly established. |
| Databáze: | OpenAIRE |
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