Globin-coupled sensors and protoglobins share a common signaling mechanism
| Autor: | Xuehua Wan, Shaobin Hou, Jennifer A. Saito, Maqsudul Alam, Kit Shan Lee |
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| Jazyk: | angličtina |
| Předmět: |
Archaeal Proteins
Recombinant Fusion Proteins Biophysics Biology Signal transduction Arginine Biochemistry 03 medical and health sciences Protein structure Bacterial Proteins Structural Biology Escherichia coli Genetics Globin Receptor Molecular Biology 030304 developmental biology Regulation of gene expression 0303 health sciences 030306 microbiology Chemotaxis Pgb Cell Biology GCS Globins Protein Structure Tertiary Cell biology Oxygen Chimeric receptor Oxygen binding Function (biology) |
| Zdroj: | FEBS Letters. (13):1840-1846 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2008.05.004 |
| Popis: | The globin-coupled sensors (GCSs) and protoglobins (Pgbs) form one lineage of the globin superfamily. The GCSs are multidomain sensory proteins involved in aerotaxis or gene regulation, while the Pgbs are single-domain globins of yet unknown function. We postulate that the GCSs and Pgbs share a common signaling mechanism to modulate diverse physiological functions. To elucidate the signaling properties of individual globin domains, we constructed and expressed chimeric receptors in Escherichia coli. We demonstrate that all the chimeric receptors reversibly bind oxygen in vitro and trigger aerotactic responses in vivo. Thus, oxygen binding to the globin domains of diverse GCSs and Pgbs form a common signaling state that can trigger aerotactic responses. |
| Databáze: | OpenAIRE |
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