Expression of GSK3β, PICK1, NEFL, C4, NKCC1 and Synaptophysin in peripheral blood mononuclear cells of the first-episode schizophrenia patients
| Autor: | Xuyi Wang, Yamei Tang, Kai Huang, Shan Ding, Xiudeng Yang, Yong Liu, Bing Lang, Tingting zhang |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Synaptophysin Peripheral blood mononuclear cell 03 medical and health sciences 0302 clinical medicine Neurofilament Proteins GSK-3 Internal medicine Humans Solute Carrier Family 12 Member 2 Medicine General Psychology Messenger RNA Complement component 4 Glycogen Synthase Kinase 3 beta Neuronal Plasticity biology business.industry Nuclear Proteins Complement C4 General Medicine 030227 psychiatry Psychiatry and Mental health Real-time polymerase chain reaction Endocrinology Synaptic plasticity Leukocytes Mononuclear Schizophrenia biology.protein Female Carrier Proteins PICK1 business 030217 neurology & neurosurgery |
| Zdroj: | Asian Journal of Psychiatry. 55:102520 |
| ISSN: | 1876-2018 |
| DOI: | 10.1016/j.ajp.2020.102520 |
| Popis: | Schizophrenia (SZ) is a severe neurodevelopmental disease with unknown pathogenic mechanisms characterized with impaired cognitive function. The disturbed synaptic plasticity and synaptic loss have been widely reported in SZ. In this study, 41 first-episode schizophrenia (FES) patients and 44 healthy controls (HC) were recruited and the expression of six genes commonly relevant to synaptic functions was examined in the peripheral blood mononuclear cells (PBMCs). These genes were glycogen synthase kinase 3β (GSK3β), protein interacting with C-kinase 1 (PICK1), synaptophysin (SYP), neurofilament light (NEFL), complement component 4 (C4) and Na+-K--2Cl- cotransporter 1 (NKCC1). Real-time quantitative polymerase chain reaction (qPCR) was performed to determine the quantity of individual mRNA template. Compared to HC, the expression of PICK1 and NKCC1 genes in FES patients was relatively lower whereas the expression of NEFL was higher. No difference for the mRNA expression of GSK3β, SYP and C4 genes was detected between FES patients and HC, nor was the gender difference; Interestingly, the mRNA expression of PICK1 in female FES patients was significantly decreased compared to female HC, but not in males; and the NEFL gene was up-regulated in male FES patients but not in females. Our findings support an abnormal expression profile of synapse-related genes in the PBMCs of FES patients. |
| Databáze: | OpenAIRE |
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