[18F]DPA-714 PET imaging shows immunomodulatory effect of intravenous administration of bone marrow stromal cells after transient focal ischemia
| Autor: | Zifeng Wang, Yuji Kuge, Hironobu Yasui, Songji Zhao, Nagara Tamaki, Naoyuki Ukon, Masahito Kawabori, Hideo Shichinohe, Kiyohiro Houkin, Kei Higashikawa, Takeo Abumiya, Chengbo Tan, Ken Kazumata, Naoki Nakayama |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
lcsh:Medical physics. Medical radiology. Nuclear medicine Pathology medicine.medical_specialty Stromal cell lcsh:R895-920 Spleen Inflammation Context (language use) Standardized uptake value [18F]DPA-714 PET 03 medical and health sciences 0302 clinical medicine medicine Translocator protein Radiology Nuclear Medicine and imaging cardiovascular diseases Stroke Bone marrow stromal cell Original Research Ischemic stroke biology business.industry medicine.disease 030104 developmental biology medicine.anatomical_structure biology.protein Bone marrow medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | EJNMMI Research EJNMMI Research, Vol 8, Iss 1, Pp 1-10 (2018) |
| ISSN: | 2191-219X |
| Popis: | Background The potential application of bone marrow stromal cell (BMSC) therapy in stroke has been anticipated due to its immunomodulatory effects. Recently, positron emission tomography (PET) with [18F]DPA-714, a translocator protein (TSPO) ligand, has become available for use as a neural inflammatory indicator. We aimed to evaluate the effects of BMSC administration after transient middle cerebral artery occlusion (MCAO) using [18F]DPA-714 PET. The BMSCs or vehicle were administered intravenously to rat MCAO models at 3 h after the insult. Neurological deficits, body weight, infarct volume, and histology were analyzed. [18F]DPA-714 PET was performed 3 and 10 days after MCAO. Results Rats had severe neurological deficits and body weight loss after MCAO. Cell administration ameliorated these effects as well as the infarct volume. Although weight loss occurred in the spleen and thymus, cell administration suppressed it. In both vehicle and BMSC groups, [18F]DPA-714 PET showed a high standardized uptake value (SUV) around the ischemic area 3 days after MCAO. Although SUV was increased further 10 days after MCAO in both groups, the increase was inhibited in the BMSC group, significantly. Histological analysis showed that an inflammatory reaction occurred in the lymphoid organs and brain after MCAO, which was suppressed in the BMSC group. Conclusions The present results suggest that BMSC therapy could be effective in ischemic stroke due to modulation of systemic inflammatory responses. The [18F]DPA-714 PET/CT system can accurately demonstrate brain inflammation and evaluate the BMSC therapeutic effect in an imaging context. It has great potential for clinical application. |
| Databáze: | OpenAIRE |
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