Effect of Addition of Concentrated Proteins and Seminal Plasma Low Molecular Weight Proteins in Freezing and Thawing of Equine Semen

Autor: Guilherme Souza, Bruno Fagundes, M F Van Tilburg, José Renato Costa Caiado, Marcus Antônio Pessanha Barreto, José Frederico Straggiotti Silva
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Acta Biomedica Brasiliensia, Vol 2, Iss 1, Pp 1-7 (2011)
ISSN: 2236-0867
Popis: Difficulties in obtaining equine frozen semen with potential fertility are recognized. This study was designed to investigate the effect of seminal plasma on frozen/thawing of eight stallion semen from different breed using the following treatments: Seminal plasma with ten-fold concentrated proteins with molecular weight above 10 kDa on frozen extender; Part of seminal plasma with proteins under 10 kDa on frozen extender; Conventional freezing, using whole seminal plasma on frozen extender. Using the parameter of 30% of seminal motility post-thawing as index of good freezability, it was verified an increased percentage of stallions that presented good freezability when semen was frozen with seminal plasma containing ten-fold concentrated proteins with molecular weight above 10 kDa on frozen extender. These results, suggested the use of seminal plasma concentrated proteins from own stallion to freezing/thawing semen.
Databáze: OpenAIRE