Exploiting Knowledge of R/Avr Genes to Rapidly Clone a New LZ-NBS-LRR Family of Late Blight Resistance Genes from Potato Linkage Group IV

Autor: Juan Carlos Correa Morales, Marjon Arens, Carolien Ruyter-Spira, Edwin A. G. van der Vossen, Richard G. F. Visser, Anoma Lokossou, Steve C Whisson, Paul R. J. Birch, Gert van Arkel, Tae-Ho Park, Evert Jacobsen
Rok vydání: 2009
Předmět:
Leucine zipper
disease resistance
quantitative resistance
Phytophthora infestans
Physiology
Molecular Sequence Data
Nicotiana benthamiana
Locus (genetics)
Sequence alignment
arabidopsis-thaliana
broad-spectrum resistance
oomycete effectors
plant immune-system
Laboratorium voor Plantenveredeling
r-gene
PRI Biodiversiteit en Veredeling
solanum-bulbocastanum
Tobacco
Solanum bulbocastanum
Amino Acid Sequence
Cloning
Molecular
Gene
Alleles
Phylogeny
Plant Diseases
Plant Proteins
Solanum tuberosum
Genetics
phytophthora-infestans mont
biology
EPS-2
downy mildew
Genetic Complementation Test
fungi
food and beverages
General Medicine
R gene
Plants
Genetically Modified
biology.organism_classification
Immunity
Innate
PRI Bioscience
PRI Biodiversity and Breeding
Plant Breeding
Multigene Family
Sequence Alignment
Agronomy and Crop Science
Zdroj: Molecular Plant-Microbe Interactions, 22(6), 630-641
Molecular Plant-Microbe Interactions 22 (2009) 6
ISSN: 1943-7706
0894-0282
Popis: In addition to the resistance to Phytophthora infestans (Rpi) genes Rpi-blb1 and Rpi-blb2, Solanum bulbocastanum appears to harbor Rpi-blb3 located at a major late blight resistance locus on LG IV, which also harbors Rpi-abpt, R2, R2-like, and Rpi-mcd1 in other Solanum spp. Here, we report the cloning and functional analyses of four Rpi genes, using a map-based cloning approach, allele-mining strategy, Gateway technology, and transient complementation assays in Nicotiana benthamiana. Rpi-blb3, Rpi-abpt, R2, and R2-like contain all signature sequences characteristic of leucine zipper nucleotide binding site leucine-rich repeat (LZ-NBS-LRR) proteins, and share amino-acid sequences 34.9% similar to RPP13 from Arabidopsis thaliana. The LRR domains of all four Rpi proteins are highly homologous whereas LZ and NBS domains are more polymorphic, those of R2 being the most divergent. Clear blocks of sequence affiliation between the four functional resistance proteins and those encoded by additional Rpi-blb3 gene homologs suggest exchange of LZ, NBS, and LRR domains, underlining the modular nature of these proteins. All four Rpi genes recognize the recently identified RXLR effector PiAVR2.
Databáze: OpenAIRE
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