Laboratory automation reduces time to report of positive blood cultures and improves management of patients with bloodstream infection
| Autor: | Antonella Mencacci, Giuseppe Rizza, Francesco Donato, Elio Cenci, Alessandra Belati, Chiara Gabrielli, Martina Savoia, Antonella Repetto, Giuseppe Vittorio De Socio, Riccardo Paggi |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) medicine.medical_specialty BD Kiestra Time Factors 030106 microbiology Bacteremia Microbial Sensitivity Tests Bacterial Physiological Phenomena Gastroenterology Work cell 03 medical and health sciences Blood culture Bloodstream infections Laboratory automation Time to report Internal medicine Bloodstream infection medicine Humans Incubation Automation Laboratory medicine.diagnostic_test Bacteria business.industry Mortality rate General Medicine Anti-Bacterial Agents Bacterial Typing Techniques Infectious Diseases Early Diagnosis Blood Culture Subculture (biology) Digital reading business Empiric therapy |
| Zdroj: | European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 37(12) |
| ISSN: | 1435-4373 |
| Popis: | The impact on time to results (TTR) and clinical decisions was evaluated for mono-microbial positive blood cultures (BC) processed using the BD Kiestra Work Cell Automation (WCA) system. Positive BC were processed by the WCA system by full-automatic subculture on solid media and digital imaging after 8 h of incubation (8-h method) followed by identification (ID) and antimicrobial susceptibility testing (AST). To evaluate the accuracy of the 8-h method, ID and AST from 8-h and overnight incubated colonies were compared for the same organisms. To evaluate its clinical impact, results from 102 BC processed by the 8-h method (cases) were compared with those from 100 BC processed by overnight incubation method (controls) in a comparable period. Identification after 8-h and overnight incubation gave concordant results in 101/102 (99.0%) isolates. Among a total of 1379 microorganism-antimicrobial combinations, categorical agreement was 99.4% (1371/1379); no very major error, 7 major errors, and one minor error were observed. TTR in cases (32.8 h ± 8.3 h) was significantly (p |
| Databáze: | OpenAIRE |
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