Optimizing the extraction of antibacterial compounds from pineapple leaf fiber
| Autor: | Jin Zhang, Deng Ganran, Zhuang Zhikai, Li Mingfu, Ou Zhongqing, He Junyan, Huang Tao, Wenwei Lian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Chromatography
General Immunology and Microbiology QH301-705.5 General Neuroscience Extraction (chemistry) 02 engineering and technology Biology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences General Biochemistry Genetics and Molecular Biology 0104 chemical sciences antibacterial component pineapple leaf fiber Fiber escherichia coli orthogonal experiment Biology (General) 0210 nano-technology General Agricultural and Biological Sciences |
| Zdroj: | Open Life Sciences, Vol 11, Iss 1, Pp 391-395 (2016) |
| ISSN: | 2391-5412 |
| Popis: | Five different solvents (petroleum ether, chloroform, ethyl acetate, acetone, and distilled water) were used to extract antibacterial compounds from pineapple leaf fiber. Compounds extracted using acetone showed the greatest antibacterial effect against Escherichia coli, measured by inhibition zone diameter. Three extraction parameters including temperature, time and solid-liquid ratio were optimized through orthogonal experiment based on single factor investigations for achieving maximum active substance extraction rate and bacteriostatic effect. Results showed that using acetone, the optimum extraction conditions for temperature, time and solid-liquid ratio were 45°C, 8 h, and 1:40 (g/ml), respectively. |
| Databáze: | OpenAIRE |
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