Ectopic Expression of VvSUC27 Induces Stenospermocarpy and Sugar Accumulation in Tomato Fruits
| Autor: | Zhefang Deng, Jing Yan, Wenrui Tu, Ling Yin, Han Gao, Jinxu Xu, Nan Zhang, Yali Zhang, Qingyong Meng, Lei Zhu, Jie Wang, Yumeng Cai, Wenjie Dong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Sucrose fungi stenospermocarpy food and beverages Plant culture Plant Science Carbohydrate metabolism Biology tomato SB1-1110 Horticulture chemistry.chemical_compound chemistry Anthesis Auxin Vitis vinifera Stenospermocarpy VvSUC Petal Ectopic expression Sugar transcriptomic analysis overexpression |
| Zdroj: | Frontiers in Plant Science, Vol 12 (2021) |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2021.759047 |
| Popis: | Seedless fruits are favorable in the market because of their ease of manipulation. Sucrose transporters (SUTs or SUCs) are essential for carbohydrate metabolism in plants. Whether SUTs participate directly in causing stenospermocarpy, thereby increasing fruit quality, remains unclear. Three SUTs, namely, VvSUC11, VvSUC12, and VvSUC27 from Vitis vinifera, were characterized and ectopic expression in tomatoes. VvSUC11- and VvSUC12-overexpressing lines had similar flower and fruit phenotypes compared with those of the wild type. VvSUC27-overexpressing lines produced longer petals and pistils, an abnormal stigma, much less and shrunken pollen, and firmer seedless fruits. Moreover, produced fruits from all VvSUC-overexpressing lines had a higher soluble solid content and sugar concentration. Transcriptomic analysis revealed more genes associated with carbohydrate metabolism and sugar transport and showed downregulation of auxin- and ethylene-related signaling pathways during early fruit development in VvSUC27-overexpressing lines relative to that of the wild type. Our findings demonstrated that stenospermocarpy can be induced by overexpression of VvSUC27 through a consequential reduction in nutrient delivery to pollen at anthesis, with a subsequent downregulation of the genes involved in carbohydrate metabolism and hormone signaling. These commercially desirable results provide a new strategy for bioengineering stenospermocarpy in tomatoes and in other fruit plants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|