Enhanced polyhydroxyalkanoate (PHA) production from the organic fraction of municipal solid waste by using mixed microbial culture
| Autor: | Giuliana D'Imporzano, Michele Pognani, Bianca Colombo, Cesare Cosentino, Tommy Pepè Sciarria, Francesca Favini, Giorgio Eisele, Anna Alekseeva, Barbara Scaglia, Fabrizio Adani |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Acidogenesis Municipal solid waste Mixed microbial culture Sequencing batch reactor 010501 environmental sciences Management Monitoring Policy and Law Polyhydroxyalkanoate 01 natural sciences Applied Microbiology and Biotechnology Polyhydroxyalkanoates 010608 biotechnology 0105 earth and related environmental sciences Renewable Energy Sustainability and the Environment business.industry Chemistry Research Biodegradable waste Total dissolved solids Pulp and paper industry Biotechnology Food waste General Energy Fermentation Aerobic dynamic feeding strategy Anaerobic percolation biocell reactor business |
| Zdroj: | Biotechnology for Biofuels |
| ISSN: | 1754-6834 |
| Popis: | Background In Europe, almost 87.6 million tonnes of food waste are produced. Despite the high biological value of food waste, traditional management solutions do not consider it as a precious resource. Many studies have reported the use of food waste for the production of high added value molecules. Polyhydroxyalkanoates (PHAs) represent a class of interesting bio-polyesters accumulated by different bacterial cells, and has been proposed for production from the organic fraction of municipal solid waste (OFMSW). Nevertheless, until now, no attention has been paid to the entire biological process leading to the transformation of food waste to organic acids (OA) and then to PHA, getting high PHA yield per food waste unit. In particular, the acid-generating process needs to be optimized, maximizing OA production from OFMSW. To do so, a pilot-scale Anaerobic Percolation Biocell Reactor (100 L in volume) was used to produce an OA-rich percolate from OFMSW which was used subsequently to produce PHA. Results The optimized acidogenic process resulted in an OA production of 151 g kg−1 from fresh OFMSW. The subsequent optimization of PHA production from OA gave a PHA production, on average, of 223 ± 28 g kg−1 total OA fed. Total mass balance indicated, for the best case studied, a PHA production per OFMSW weight unit of 33.22 ± 4.2 g kg−1 from fresh OFMSW, corresponding to 114.4 ± 14.5 g kg−1 of total solids from OFMSW. PHA composition revealed a hydroxybutyrate/hydroxyvalerate (%) ratio of 53/47 and Mw of 8∙105 kDa with a low polydispersity index, i.e. 1.4. Conclusions This work showed how by optimizing acidic fermentation it could be possible to get a large amount of OA from OFMSW to be then transformed into PHA. This step is important as it greatly affects the total final PHA yield. Data obtained in this work can be useful as the starting point for considering the economic feasibility of PHA production from OFMSW by using mixed culture. Electronic supplementary material The online version of this article (doi:10.1186/s13068-017-0888-8) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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