Purification, cDNA structure and biological significance of a single insulin-like growth factor-binding domain protein (SIBD-1) identified in the hemocytes of the spider Cupiennius salei
| Autor: | Tommy Baumann, Sathyan Chandru, Carlo R. Largiadèr, Johann Schaller, Stefan Schürch, Wolfgang Nentwig, Lucia Kuhn-Nentwig, Kathrin Streitberger, Urs Kämpfer |
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| Rok vydání: | 2011 |
| Předmět: |
Hemocytes
Molecular Sequence Data Protein domain Peptide Real-Time Polymerase Chain Reaction Biochemistry Arthropod Proteins 03 medical and health sciences Rapid amplification of cDNA ends Complementary DNA Animals Insulin-like growth factor binding Amino Acid Sequence Cloning Molecular Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Base Sequence Sequence Homology Amino Acid biology Edman degradation 030302 biochemistry & molecular biology Spiders biology.organism_classification Cupiennius salei Molecular biology Protein Structure Tertiary 3. Good health Amino acid Insulin-Like Growth Factor Binding Proteins chemistry Insect Science Female Sequence Alignment |
| Zdroj: | Insect Biochemistry and Molecular Biology. 41:891-901 |
| ISSN: | 0965-1748 |
| DOI: | 10.1016/j.ibmb.2011.08.003 |
| Popis: | Cupiennius salei single insulin-like growth factor-binding domain protein (SIBD-1), which exhibits an IGFBP N-terminal domain-like profile, was identified in the hemocytes of the spider C. salei. SIBD-1 was purified by RP-HPLC and the sequence determined by a combination of Edman degradation and 5'-3'- RACE PCR. The peptide (8676.08 Da) is composed of 78 amino acids, contains six intrachain disulphide bridges and carries a modified Thr residue at position 2. SIBD-1 mRNA expression was detected by quantitative real-time PCR mainly in hemocytes, but also in the subesophageal nerve mass and muscle. After infection, the SIBD-1 content in the hemocytes decreases and, simultaneously, the temporal SIBD-1 expression seems to be down-regulated. Two further peptides, SIBD-2 and IGFBP-rP1, also exhibiting IGFBP N-terminal domain variants with unknown functions, were identified on cDNA level in spider hemocytes and venom glands. We conclude that SIBD-1 may play an important role in the immune system of spiders. |
| Databáze: | OpenAIRE |
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