Rosette-forming cell and cytophilic antibody
| Autor: | J. L. Birrien, Bach Jf, C. Bleux, P. Liacopoulos, J. Couderc |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Male
Erythrocytes Time Factors Immunology Cell Population Hemolytic Plaque Technique Spleen Antibodies Rosette (zoology) Mice Cell Adhesion medicine Animals Immunology and Allergy Lymphocytes Antigens Columbidae education Incubation Immunity Cellular Mice Inbred C3H education.field_of_study Sheep biology Immune Sera Temperature Cytophilic antibody Molecular biology Immune Adherence Reaction medicine.anatomical_structure Immunization biology.protein Antibody Plastics |
| Zdroj: | European Journal of Immunology. 3:310-313 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.1830030511 |
| Popis: | Immunization of mice with two non-cross-reacting erythrocytes results in the appearance of double rosettes. Injection of sera from doubly immunized animals into normal mice also results in double rosette appearance when spleen cells are examined after rosette formation and incubation at 0 °C for 1 h. However, passive rosettes entirely disappear after overnight incubation at 4 °C or after incubation for 1 h at 37 °C, whereas active ones persist in significant numbers. These treatments therefore provide an accurate discrimination between cells passively sensitized by cytophilic antibody and cells actively forming antibody. Removal of cells adhering to plastic surfaces from spleen cells of doubly immunized animals provokes a decrease of simple rosettes and a disappearance of doubles among non-adherent cells. When adherent cells are tested for existence of hemolytic plaque-forming cells (PFC), it was found that PFC also adhere. Conversely, the non-adherent cell population is severely depleted of such cells. Moreover, double PFC, again found in initial cell populations, were recovered with adherent cells but were absent in the final non-adherent population. |
| Databáze: | OpenAIRE |
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