Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster resonance energy transfer
| Autor: | Skajaa, T., Zhao, Y., van den Heuvel, D.J., Gerritsen, H.C., Cormode, D.P., Koole, R., van Schooneveld, M.M., Post, J.A., Fisher, E.A., Fayad, Z.A., de Mello Donega, C., Meijerink, A., Mulder, W.J.M., Biomolecular Imaging, Condensed Matter and Interfaces, Inorganic Chemistry and Catalysis, Soft Condensed Matter and Biophysics, Sub Condensed Matter and Interfaces, Sub Molecular Biophysics, Dep Scheikunde, Sub Inorganic Chemistry and Catalysis, Sub Biomolecular Imaging |
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| Přispěvatelé: | Medical Biochemistry |
| Rok vydání: | 2010 |
| Předmět: |
A lipoprotein
Chemistry Mechanical Engineering Energy transfer Lipoproteins technology industry and agriculture Nanoparticle Bioengineering General Chemistry Plasma protein binding Condensed Matter Physics Article Förster resonance energy transfer Nuclear magnetic resonance Quantum dot Live cell imaging Quantum Dots Biophysics Fluorescence Resonance Energy Transfer Nanoparticles General Materials Science lipids (amino acids peptides and proteins) Lipoprotein Protein Binding |
| Zdroj: | Nano letters, 10(12), 5131-5138. American Chemical Society Nano Letters, 10, 5131. American Chemical Society Skajaa, T, Zhao, Y, van den Heuvel, D J, Gerritsen, H C, Cormode, D P, Koole, R, van Schooneveld, M M, Post, J A, Fisher, E A, Fayad, Z A, de Mello Donega, C, Meijerink, A & Mulder, W J M 2010, ' Quantum Dot and Cy5.5 Labeled Nanoparticles to Investigate Lipoprotein Biointeractions via Förster Resonance Energy Transfer ' Nano Letters . https://doi.org/10.1021/nl1037903 |
| ISSN: | 1530-6992 1530-6984 |
| Popis: | The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Förster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein-lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells |
| Databáze: | OpenAIRE |
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