Determination of the 3D Genome Organization of Bacteria Using Hi-C
| Autor: | Frédéric Crémazy, Remus T. Dame, David C. Grainger, Lisa E Lamberte, James R. J. Haycocks, Fatema-Zahra M Rashid |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chromosome Bacterial genome size Computational biology Biology Compartmentalization (psychology) biology.organism_classification Genome Chromosome conformation capture 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry DNA Bacteria Genomic organization |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493986743 Methods in Molecular Biology Methods in Molecular Biology, 1837, 3-18. New York, U.S.A.: Humana Press |
| DOI: | 10.1007/978-1-4939-8675-0_1 |
| Popis: | The spatial organization of genomes is based on their hierarchical compartmentalization in topological domains. There is growing evidence that bacterial genomes are organized into insulated domains similar to the Topologically Associating Domains (TADs) detected in eukaryotic cells. Chromosome conformation capture (3C) technologies are used to analyze in vivo DNA proximity based on ligation of distal DNA segments crossed-linked by bridging proteins. By combining 3C and high-throughput sequencing, the Hi-C method reveals genome-wide interactions within topological domains and global genome structure as a whole. This chapter provides detailed guidelines for the preparation of Hi-C sequencing libraries for bacteria. |
| Databáze: | OpenAIRE |
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