Tissue-specific changes in Ca2+-ATPase and Na+/K+-ATPase activities in freshwater African catfish Clarias gariepinus juvenile exposed to oxadiazon
| Autor: | Christopher Didiugwu Nwani, Ndubuisi Stanley Oluah, Ifeanyi Oscar Aguzie, Chidimma Oluah, Innocent Chinedu Ngene, Bernard Obialo Mgbenka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Clarias gariepinus
medicine.medical_specialty ATPase 010501 environmental sciences 01 natural sciences Osmoregulation Clarias Internal medicine lcsh:Zoology medicine Bioassay lcsh:QL1-991 Na+/K+-ATPase 0105 earth and related environmental sciences Ionic imbalance chemistry.chemical_classification Kidney biology General Engineering 04 agricultural and veterinary sciences biology.organism_classification medicine.anatomical_structure Enzyme Endocrinology chemistry 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Herbicide Ca2+-ATPase Homeostasis Catfish |
| Zdroj: | Journal of Basic and Applied Zoology, Vol 81, Iss 1, Pp 1-12 (2020) |
| DOI: | 10.1186/s41936-020-00186-8 |
| Popis: | BackgroundThis study investigated the effect of sublethal concentrations (0.0, 0.3, 0.6, and 1.2 mg·L-1) of herbicide oxadiazon (ODZ) on the activities of Ca2+-ATPase and Na+/K+-ATPase in juvenileClarias gariepinus.MethodsOne hundred eighty juveniles ofClarias gariepinus(mean weight 58.88 ± 1.24 g and mean length 22.34 ± 1.52 cm) were randomly divided into four groups and exposed to sublethal concentrations (0.00, 0.3, 0.6, and 1.2 mg·L-1ODZ) for 21 days in a static renewal bioassay system in which the herbicide and water were replaced completely every day. The changes in Ca2+- and Na+/K+-ATPase activities in the gill, kidney, liver, and heart of the fish were assayed on days 1, 7, 14, and 21.ResultThe result showed significant alteration in the activity of these membrane-bound enzymes. There was duration and concentration-dependent significant (p 2+-ATPase activity in the treatment groups when compared with the control. The Na+/K+-ATPase activity was significantly (p 2+-ATPase and Na+/K+-ATPase in this study may be indication of impaired ionic transport and imbalance in the fish which may trigger other biochemical, physiological, and even neurological consequences that may compromise several body functions.ConclusionThe alteration of the ATPase activities inC. gariepinusby ODZ is likely to affect the ATP usage and energy metabolism in the fish serious repercussions on Ca2+homeostasis, Na+/K+sodium pump, and Ca2+/Na+exchanger The results suggested that assay of the enzymes could be used as a biomarker of water pollution. |
| Databáze: | OpenAIRE |
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