Immunoregulatory effects of 4-(4-chlorophenyl)-1-(5-amino-3-methylisoxazole-4-carbonyl)-thiosemicarbazide (06K) in non-immunized and SRBC-immunized mice
| Autor: | Ewa Zaczyńska, Michał Zimecki, Angelika Drynda, Marcin Mączyński, Stanisław Ryng, Bożena Obmińska-Mrukowicz |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Erythrocytes Ovalbumin Pharmaceutical Science Carrageenan T-Lymphocytes Regulatory 03 medical and health sciences 0302 clinical medicine Immune system Antigen Animals Immunologic Factors Hypersensitivity Delayed IL-2 receptor Lymphopoiesis Pharmacology Inflammation Immunity Cellular Mice Inbred BALB C Sheep biology Dose-Response Relationship Drug FOXP3 Immunity Humoral Semicarbazides Disease Models Animal 030104 developmental biology Phenotype 030220 oncology & carcinogenesis Immunology biology.protein Mice Inbred CBA Immunization Lymph CD8 Spleen |
| Zdroj: | The Journal of pharmacy and pharmacology. 68(12) |
| ISSN: | 2042-7158 |
| Popis: | Objectives Immunoregulatory properties of 06K derivative (4-(4-chlorophenyl)-1-(5-amino-3-methylisoxazole-4-carbonyl)-thiosemicarbazide) in mouse in vivo models were investigated. Methods Several in vivo models were used: humoral and cellular immune response, carrageenan inflammatory reaction and determination of lymphocyte subsets in non-immunized mice. Key findings The compound administered before or after immunization with sheep erythrocytes (sheep red blood cell (SRBC)) elevated the number of plaque-forming cells (PFC), and this effect was stronger at lower doses. Although total haemagglutinin titres to SRBC decreased upon postimmunization treatment, IgG titre increased. In the model of delayed-type hypersensitivity (DTH) to ovalbumin (OVA), the compound, applied intraperitoneally before an eliciting dose of an antigen but not before immunization, inhibited the magnitude of a cutaneous reaction. Further, 06K significantly diminished carrageenan-induced foot pad inflammation when administered 1 h before carrageenan. The compound, administered intraperitoneally to naïve mice, elicited changes in weight, cell number in lymphoid organs and content of lymphocyte subsets, depending on the dose and number of applications. Phenotypic changes included increased turnover of thymocytes, changes in B-cell distribution in spleens and lymph nodes, increased percentage of CD8+ cells and regulatory CD4+CD25+ Foxp3+ T cells. Conclusions Immunoregulatory properties of 06K involve mobilization of lymphopoiesis and generation of regulatory T cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text