Epithelial microchimerism: consistent finding in human liver transplants
| Autor: | Mario Rizzetto, Antonina Smedile, Luciano Gubetta, Elisa Bacillo, Silvia Gaia, Susanna Cappia, Ezio Gaia |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Pathology medicine.medical_specialty medicine.medical_treatment Biopsy Biology Liver transplantation Chimerism Polyploidy medicine Humans Lobules of liver In Situ Hybridization Fluorescence Aged Retrospective Studies Chromosomes Human X Chromosomes Human Y Hepatology medicine.diagnostic_test Gastroenterology Microchimerism Cell Differentiation Epithelial Cells Middle Aged Immunohistochemistry Liver Transplantation Hepatic stellate cell Hepatocytes Female Stem cell Fluorescence in situ hybridization Follow-Up Studies |
| Zdroj: | Journal of gastroenterology and hepatology. 21(12) |
| ISSN: | 0815-9319 |
| Popis: | Background: Eleven liver biopsies from six male patients who received a liver transplant (LT) from female donors were examined in order to determine whether male host-derived hepatic cells were present in female grafts that exhibited minimal or important inflammatory damage. Methods: Immunohistochemistry for epithelial cell type differentiation (anticytokeratin monoclonal antibody) and fluorescence in situ hybridization for XY chromosomes identification were performed on each slide. Results: Host-derived hepatic cells were found in all except one transplant, with a frequency ranging from 2.3 to 25‰ of the total hepatocytes in the biopsy specimen. They were usually found as isolated cells scattered throughout the hepatic lobule; in one patient they were grouped into little clusters. Host-derived hepatic cells persisted throughout the histological follow up (up to 535 days after LT). Polyploidy for XY chromosome was observed. Conclusion: Hepatocytes derived from extra-hepatic stem cells are frequently found in small numbers in human liver grafts and persist over time. |
| Databáze: | OpenAIRE |
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