Dynamic in-vivo imaging of dual-triggered microspheres for sustained release applications: Synthesis, characterization and cytotoxicity study
| Autor: | George Loudos, Lazaros Palamaris, Eirini Fragogeorgi, Nikos Boukos, Christos Tapeinos, Alexandros Chatzipavlidis, George Kordas, Eleni K. Efthimiadou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Biodistribution
Vinyl Compounds Polymers Drug Compounding Pharmaceutical Science Nanotechnology chemistry.chemical_compound Magnetics Mice Drug Delivery Systems In vivo Animals Humans Polymethyl Methacrylate Tissue Distribution Methyl methacrylate Acrylic acid Drug Carriers Antibiotics Antineoplastic Hydrogen-Ion Concentration Controlled release Poly(methyl methacrylate) Microspheres Cross-Linking Reagents HEK293 Cells Magnetic Fields chemistry Acrylates Doxorubicin visual_art Delayed-Action Preparations Drug delivery Injections Intravenous visual_art.visual_art_medium Biophysics Magnetic nanoparticles Nanoparticles Female |
| Zdroj: | International Journal of Pharmaceutics |
| ISSN: | 0378-5173 |
| DOI: | 10.1016/j.ijpharm.2013.11.037 |
| Popis: | This paper deals with the synthesis, characterization and property evaluation of drug-loaded magnetic microspheres with pH-responsive cross-linked polymer shell. The synthetic procedure consists of 3 steps, of which the first two comprise the synthesis of a poly methyl methacrylate (PMMA) template and the synthesis of a shell by using acrylic acid (AA) and methyl methacrylate (MMA) as monomers, and divinyl benzene (DVB) as cross-linker. The third step of the procedure refers to the formation of magnetic nanoparticles on the microsphere's surface. AA that attaches pH-sensitivity in the microspheres and magnetic nanoparticles in the inner and the outer surface of the microspheres, enhance the efficacy of this intelligent drug delivery system (DDS), which constitutes a promising approach toward cancer therapy. A number of experimental techniques were used to characterize the resulting microspheres. In order to investigate the in vitro controlled release behavior of the synthesized microspheres, we studied the Dox release percentage under different pH conditions and under external magnetic field. Hyperthermia caused by an alternating magnetic field (AFM) is used in order to study the doxorubicin (Dox) release behavior from microspheres with pH functionality. The in vivo fate of these hybrid-microspheres was tracked by labeling them with the γ-emitting radioisotope (99m)Tc after being intravenously injected in normal mice. According to our results, microsphere present a pH depending and a magnetic heating, release behavior. As expected, labeled microspheres were mainly found in the mononuclear phagocyte system (MPS). The highlights of the current research are: (i) to illustrate the advantages of controlled release by combining hyperthermia and pH-sensitivity and (ii) to provide noninvasive, in vivo information on the spatiotemporal biodistribution of these microsphere by dynamic γ-imaging. |
| Databáze: | OpenAIRE |
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