RNA Recombination Enhances Adaptability and Is Required for Virus Spread and Virulence
| Autor: | Simone Bianco, Raul Andino, Leonid Brodsky, Elizabeth Faul Goldstein, Ashley Acevedo, Igor M. Rouzine, Mikhail Farkov, Yinghong Xiao |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Mutation rate Virus Replication 2.2 Factors relating to the physical environment Viral Aetiology media_common Genetics Recombination Genetic education.field_of_study Virulence Clonal interference DNA-Directed RNA Polymerases Adaptation Physiological Poliovirus Infectious Diseases Medical Microbiology Mutation (genetic algorithm) RNA Viral Infection Recombination Biotechnology Evolution media_common.quotation_subject Physiological 030106 microbiology Population Immunology Biology Microbiology Adaptability Virus Article Evolution Molecular 03 medical and health sciences Viral Proteins Genetic Virology Animals Humans Adaptation Selection Genetic education Selection Molecular 030104 developmental biology Emerging Infectious Diseases Good Health and Well Being Viral replication RNA Parasitology Poliomyelitis |
| Zdroj: | Cell host & microbe, vol 19, iss 4 |
| ISSN: | 1934-6069 |
| Popis: | Mutation and recombination are central processes driving microbial evolution. A high mutation rate fuels adaptation, but also generates deleterious mutations. Recombination between two different genomes may resolve this paradox, alleviating effects of clonal interference and purging deleterious mutations. Here we demonstrate that recombination significantly accelerates adaptation and evolution during acute virus infection. We identified a poliovirus recombination determinant within the virus polymerase, mutation of which reduces recombination rates without altering replication fidelity. By generating a panel of variants with distinct mutation rates and recombination ability, we demonstrate that recombination is essential to enrich the population in beneficial mutations and purge it from deleterious mutations. The concerted activities of mutation and recombination are key to virus spread and virulence in infected animals. These findings inform a mathematical model to demonstrate that poliovirus adapts most rapidly at an optimal mutation rate determined by the trade-off between selection and accumulation of detrimental mutations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|