Detection of Cry1Ab toxin in the leaves of MON 810 transgenic maize
| Autor: | Eszter Takács, Éva Lauber, András Székács, Béla Darvas |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Genetically modified maize
Bacillus thuringiensis Toxins MON 810 Phenology Toxin fungi Significant difference Fresh weight food and beverages Enzyme-Linked Immunosorbent Assay Genetically modified crops Biology Plants Genetically Modified medicine.disease_cause Zea mays Biochemistry Analytical Chemistry Endotoxins Plant Leaves Hemolysin Proteins Animal science Bacterial Proteins medicine Leaf weight |
| Zdroj: | Analytical and Bioanalytical Chemistry. 396:2203-2211 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-009-3384-6 |
| Popis: | The distribution of Cry1Ab toxin was detected in the leaves of genetically modified maize of genetic event MON 810 by enzyme-linked immunosorbent assay. Cry1Ab toxin contents in the leaves at reproductive (milk, R3) phenological stage were measured to be between 3,878 and 11,148 ng Cry1Ab toxin/g fresh weight. Toxin content was significantly lesser (significant difference (SD) = 1,823 ng Cry1Ab toxin/g fresh leaf weight, p < 0.01) in leaves at the lowest leaf level, than at higher leaf levels, probably due to partial leaf necrotisation. A substantial (up to 22%) plant-to-plant variation in Cry1Ab contents in leaves was observed. When studying toxin distribution within the cross and longitudinal sections of single leaves, lesser variability was detected diagonally, with approximately 20% higher toxin concentrations at or near the leaf vein. More significant variability (SD = 2,220 ng Cry1Ab toxin/g fresh leaf weight, p < 0.01) was seen lengthwise along the leaf, starting at 1,892 ng Cry1Ab toxin/g fresh weight at the sheath and rising to maximum concentration at the middle of the lamella. Cry1Ab toxin content may suffer significant (SD = 2,230 ng Cry1Ab toxin/g fresh leaf weight, p < 0.01) decreases in the leaf due to necrotisation. The results indicate that the longitudinal dimension of the leaf has more significance for sampling purposes than the diagonal position. |
| Databáze: | OpenAIRE |
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