Hyperimmunization of apo-E-deficient mice with homologous malondialdehyde low-density lipoprotein suppresses early atherogenesis
| Autor: | Yuri Kopolovic, Jacob George, Dror Harats, Boris Gilburd, Georg Wick, Arnon Afek, Iris Goldberg, Yehuda Shoenfeld, Hana Levkovitz, A. Shaish |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Apolipoprotein E
medicine.medical_specialty Arteriosclerosis Antibodies Lesion Hyperimmunization chemistry.chemical_compound Mice Immune system Apolipoproteins E Internal medicine Malondialdehyde medicine Animals biology Sinus of Valsalva Immunohistochemistry Lipids Lipoproteins LDL Endocrinology chemistry Low-density lipoprotein Immunology biology.protein lipids (amino acids peptides and proteins) Female Immunization medicine.symptom Antibody Cardiology and Cardiovascular Medicine Lipoprotein |
| Zdroj: | Atherosclerosis. 138(1) |
| ISSN: | 0021-9150 |
| Popis: | The role of the immune system in modulating atherosclerosis has recently been the subject of intensive research. Several previous authors have put forward a paradigm of the autoimmune process occurring in the vicinity of the plaque. Two recent studies have shown that immunization of rabbits with homologous modified low-density lipoprotein (LDL) led to suppression of atherosclerosis. In the current study we evaluated the effects of homologous malondialdehyde (MDA)-LDL immunizations on atherogenesis in apo-E-deficient mice. Two groups of female chow-diet-fed, apo-E-deficient mice (n = 10) were either immunized with homologous MDA-LDL or with phosphate buffer saline (PBS) at 2-week intervals. The mice were sacrificed 12 weeks following the primary immunization. The MDA-LDL-immunized mice were shown to develop high titers of anti-MDA-LDL antibodies. Atherosclerosis, determined by the lesion size at the aortic sinus, was significantly suppressed in the MDA-LDL-immunized mice as compared with their littermates immunized with PBS (mean area +/- S.D.; 74000 +/- 17300 microm2 versus 158000 +/- 12800 microm2; P0.01). No differences were found between the groups with respect to the cellular composition of the atherosclerotic plaques. The results of this study show that immunization with MDA-LDL has a protective effect in apo-E-deficient mice, and further suggests that this mouse model is suitable for studies of immunomodulation. |
| Databáze: | OpenAIRE |
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