T-2 and HT-2 toxins in feed and food from Southern Italy, determined by LC-MS/MS after immunoaffinity clean-up

Autor:	Mauro Esposito, Michele De Crescenzo, Grazia Guadagnuolo, Pasquale Gallo, Claudia Imperato, Valeria Urbani, Ilaria Di Marco Pisciottano, Vittorio Soprano, Samantha Imbimbo
Rok vydání:	2020
Předmět:	Food Contamination Raw material Toxicology medicine.disease_cause 01 natural sciences Chromatography Affinity 0404 agricultural biotechnology Tandem Mass Spectrometry Lc ms ms medicine media_common.cataloged_instance Animals Humans Food science European union Chromatography High Pressure Liquid media_common Chemistry Toxin 010401 analytical chemistry Public Health Environmental and Occupational Health Reproducibility of Results 04 agricultural and veterinary sciences Contamination 040401 food science Animal Feed 0104 chemical sciences Clean-up Triple quadrupole mass spectrometer T-2 Toxin Italy Food Microbiology Edible Grain Food Analysis Food Science
Zdroj:	Food additivescontaminants. Part B, Surveillance. 13(4)
ISSN:	1939-3229
Popis:	A method to determine T-2 and HT-2 toxins, both in feed and food, is described. The method is based on sample clean-up by immunoaffinity columns, followed by triple quadrupole LC-MS/MS determination. It was in-house validated in compliance with the European Union legislation, in the concentration range 5.0-750 µg kg-1 and accredited according to the ISO/IEC 17025 standard, for the scope of official control of feed and food. The method was applied to monitor the contamination levels of T-2 and HT-2 toxins in 105 feed samples, both raw materials and compound feed and 17 cereals and derived products for human consumption, collected in Southern Italy between 2015 and 2019. T-2 toxin was quantified in 32 feed (30.5%) and in 2 food samples (11.8%). HT-2 toxin was quantified in 65 feed (61.9%) and in 3 food (17.6%) samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::beeb1be6ada8bf0485ea6474970922cd https://pubmed.ncbi.nlm.nih.gov/32466723 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.