Enzymatic hydrolysis for the systematic production of second-generation glucose from the dual polysaccharide reserves of an anti-pollutant plant
| Autor: | Theofanis-Matthaios Migkos, Konstantinos Flevaris, Georgia Ioannidou, Christos Chatzidoukas, Anna Karapatsia |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Environmental Engineering Typha domingensis biology Ethanol Renewable Energy Sustainability and the Environment Chemistry Starch Hydrolysis Substrate (chemistry) Bioengineering General Medicine Polysaccharide biology.organism_classification chemistry.chemical_compound Glucose Enzymatic hydrolysis Yield (chemistry) Environmental Pollutants Food science Response surface methodology Biomass Cellulose Waste Management and Disposal |
| Zdroj: | Bioresource technology. 340 |
| ISSN: | 1873-2976 |
| Popis: | In this study, the anti-pollutant macrophyte Typha domingensis is exploited for the production of highly concentrated second-generation glucose. A two-stage starch and cellulose enzymatic hydrolysis process is compared for the first time with a single-stage simultaneous starch and cellulose hydrolysis approach, with the former achieving enhanced glucose production, making it more promising for large-scale deployment. The proposed two-stage process is optimized via the Box-Behnken response surface methodology achieving glucose yield values of 74.4% and 71.7% with respect to the starch and cellulose fraction, respectively. Elevated shaking rates are shown to exert a positive effect on both starch and cellulose enzymatic hydrolysis only under high initial substrate concentrations and high initial enzyme to substrate ratios, indicating the importance of accounting for the synergies between key process variables when aiming to increase glucose production. The findings of the presented experimental framework aspire to support future scale-up studies and techno-economic assessments. |
| Databáze: | OpenAIRE |
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