Mutational Analysis of Immunoglobulin Germline Derived Vλ4B Light Chains in Rheumatoid Arthritis
| Autor: | Wasyl Malyj, Dick L. Robbins, Thomas J. Semrad, Thomas P. Kenny |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Silent mutation
DNA Complementary DNA Mutational Analysis Molecular Sequence Data Immunology Immunoglobulin light chain medicine.disease_cause Germline Arthritis Rheumatoid Immunoglobulin lambda-Chains Antigen Polymorphism (computer science) Sequence Homology Nucleic Acid medicine Gene Rearrangement B-Lymphocyte Light Chain Humans Immunology and Allergy RNA Messenger Amino Acid Sequence Codon Gene Germ-Line Mutation Aged DNA Primers Genetics Mutation Polymorphism Genetic Base Sequence Genes Immunoglobulin Sequence Homology Amino Acid biology Middle Aged biology.protein Antibody |
| Zdroj: | Clinical Immunology. 99:283-290 |
| ISSN: | 1521-6616 |
| Popis: | In order to better characterize the expression of a family of light chains previously expressed in IgM rheumatoid factors, we studied the usage and somatic mutational pattern of the Vlambda4A light chain gene in rheumatoid arthritis (RA) patients. We sequenced 11 different transcripts of Vlambda4A from the synovial tissue of three different RA patients. For comparison, we found 8 rearranged transcripts of Vlambda4A from 4 normal peripheral blood lymphocyte libraries and 1 rearranged transcript from a non-RA con-A-resistant hybridoma in GenBank. A previously undescribed polymorphism of Vlambda4A was noted. Furthermore, conserved replacement mutations in the complementary determining regions, common silent mutations around these replacement mutations, and two subsets of mutated sequences were detected in multiple RA patients. These mutation patterns also correlated with observed consistencies in the rearrangements of the Vlambda4A/Jlambda junction. These data suggest that there is clonal expansion of Vlambda4A light chains in the RA synovium in response to a RA-specific antigen or as the result of an idiotypic response in RA. |
| Databáze: | OpenAIRE |
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