Counterimmunoelectrophoresis with influenza antigens. I. Use of avian plague virus to detect type-specific antibodies to influenza A in human sera
| Autor: | J. C. Russi-Cahill, M. H. de Peluffo, R. E. Somma-Moreira, M. C. Mogdasy |
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| Rok vydání: | 1975 |
| Předmět: |
Immunodiffusion
Guinea Pigs Chick Embryo Antibodies Viral Virus Serology Blood serum Antigen Antibody Specificity Influenza Human Immunology and Allergy Animals Humans Antigens Viral Immunoelectrophoresis biology Immune Sera Complement Fixation Tests Complement fixation test Virology Infectious Diseases Influenza A virus Immunology biology.protein Antibody Counterimmunoelectrophoresis |
| Zdroj: | The Journal of infectious diseases. 131(1) |
| ISSN: | 0022-1899 |
| Popis: | Avian plague virus was used as antigen in a counterimmunoelectrophoresis technique. This virus was selected because it detects only type-specific influenza A antibodies in human sera, avoiding the possible interference of other antigens with anodic migration. The results with reference sera, as well as the correlation of positive sera found by counterimmunoelectrophoresis and complement fixation with the proposed antigen, in the absence of other types of antibodies to fowl plague virus antigen, support the conclusion that the counterimmunoelectrophoresis technique reveals type-specific antibodies. The test is more sensitive than immunodiffusion but less sensitive than complement fixation. Its sensitivity, simplicity, and rapidity make it suitable for serologic surveys of human influenza A. Counterimmunoelectrophoresis (CIE) has been successfully used in various fields of biology. Berlin and Pirojiboot reported the application of the technique for diagnosis of influenza infection, with human strains as antigens [1]. The present work describes a modification of their technique for evaluation of the possible detection of exclusively type-specific antibodies to influenza in human sera, by the introduction of avian plague virus (FPV) as antigen. |
| Databáze: | OpenAIRE |
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