Application of microdialysis to study the in vivo release of pingyangmycin from in situ gels in rabbits
| Autor: | Li Zhang, Pingtian Ding, Zi-bin Gao, Jian Shi, Jing Wei, Dawei Chen, Zhenting Yuan |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Pharmacology
In situ Detection limit Male Microdialysis Chromatography Chemistry Hydrochloride Clinical Biochemistry Reproducibility of Results General Medicine Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Bleomycin Pharmacokinetics In vivo Drug Discovery Animals Pingyangmycin Rabbits Molecular Biology Chromatography High Pressure Liquid |
| Zdroj: | Biomedical chromatography : BMC. 20(11) |
| ISSN: | 0269-3879 |
| Popis: | Microdialysis was used together with HPLC to monitor the local concentration of pingyangmycin hydrochloride (PYM) after embolizing rabbit ear-veins with an injectable sustained release formulation, PYM-Zein/PYM-Zein-sucrose acetate isobutyrate (SAIB), in situ gel. The dialysis probe was perfused at 2 microL/min. The in vivo recovery was 46.6 3.1% (n = 4). The samples were injected directly into HPLC. PYM was detected using UV detector at 291 nm. Separation from other components in the dialysate was performed using a Discovery((R)) RP-Amide C(16) column within 12 min. The response for PYM in the dialysate was linear (r(2) > 0.996) over the range of 2.5-212 microg/mL. The limit of detection and limit of quantitation of PYM in the dialysate were 0.4 and 1.5 microg/mL, respectively. The results demonstrated that the in situ gel of PYM-Zein-SAIB could extend the release of PYM to 4 days. SAIB could significantly cut down the initial burst of PYM from the in situ gels (p < 0.05). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text