Correlation between Src family member regulation by the protein-tyrosine-phosphatase CD45 and transmembrane signaling through the T-cell receptor
| Autor: | Andrey S. Shaw, E D Cahir McFarland, T R Hurley, Matthew L. Thomas, B M Sefton, Jeanette T. Pingel |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
T-Lymphocytes
Immunoblotting Receptors Antigen T-Cell Protein tyrosine phosphatase Lymphocyte Activation Proto-Oncogene Proteins c-fyn Peptide Mapping Receptor tyrosine kinase SH3 domain chemistry.chemical_compound Proto-Oncogene Proteins Animals Cyanogen Bromide Phosphorylation Multidisciplinary biology Cell Membrane T-cell receptor Tyrosine phosphorylation Protein-Tyrosine Kinases Molecular biology Peptide Fragments Clone Cells Genes src chemistry Lymphocyte Specific Protein Tyrosine Kinase p56(lck) biology.protein Leukocyte Common Antigens Protein Tyrosine Phosphatases Signal transduction Signal Transduction Research Article Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | Proceedings of the National Academy of Sciences. 90:1402-1406 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.90.4.1402 |
| Popis: | Stimulation of tyrosine phosphorylation is an early and important event in antigen-induced T-cell activation. T-cell clones deficient in expression of CD45, a transmembrane protein-tyrosine-phosphatase (protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48), are impaired in their ability to respond to either antigen or T-cell receptor cross-linking. Analysis of the CD45-deficient CD8+ T-cell clone L3M-93 demonstrates that the Src family members p56lck and p59fyn show increased immunoreactivity with anti-phosphotyrosine antibody and exhibit decreased kinase activity. The site of increased tyrosine phosphorylation in Src family members was identified by comparison of cyanogen bromide peptide maps. Phosphorylation of the C-terminal phosphopeptide, containing the negative regulatory site of tyrosine phosphorylation, from the CD45-deficient cells was increased 8-fold for p56lck and 2-fold for p59fyn. These data suggest that CD45 dephosphorylates the negative regulatory site of multiple Src family members in the cytotoxic T-lymphocyte clone L3 and show a correlation between the ability to respond efficiently to antigen and the dephosphorylation of Src family members by CD45. |
| Databáze: | OpenAIRE |
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