Inhibition of Neoplastic Transformation and Chemically-Induced Skin Hyperplasia in Mice by Traditional Chinese Medicinal Formula Si-Wu-Tang
| Autor: | Cyrus Parsa, Nan Mei, Steven Yeung, Ying Huang, Suhui Zhang, Andy Chang, Mandy Liu, Robert Orlando, Kevin M. Huang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DMBA medicine.disease_cause Mice Inbred SENCAR NF-κB Mice 0302 clinical medicine Epidermal growth factor Genes Reporter Skin Nutrition and Dietetics biology cancer prevention skin cancer Chemistry NF-kappa B Ames test Cell Transformation Neoplastic 030220 oncology & carcinogenesis Female SWT SENCAR mice EGF AP-1 JB6 lcsh:Nutrition. Foods and food supply DNA damage NF-E2-Related Factor 2 9 10-Dimethyl-1 2-benzanthracene lcsh:TX341-641 Article 03 medical and health sciences In vivo Cell Line Tumor medicine Animals Anticarcinogenic Agents Neoplastic transformation Hyperplasia Tumor Necrosis Factor-alpha Proliferating cell nuclear antigen Transcription Factor AP-1 030104 developmental biology Gene Expression Regulation Immunology biology.protein Cancer research Tumor promotion Carcinogenesis Carrier Proteins Food Science DNA Damage Drugs Chinese Herbal |
| Zdroj: | Nutrients Nutrients, Vol 9, Iss 3, p 300 (2017) Nutrients; Volume 9; Issue 3; Pages: 300 Nutrients, 9(3):300 |
| ISSN: | 2072-6643 |
| Popis: | Exploring traditional medicines may lead to the development of low-cost and non-toxic cancer preventive agents. Si-Wu-Tang (SWT), comprising the combination of four herbs, Rehmanniae, Angelica, Chuanxiong, and Paeoniae, is one of the most popular traditional Chinese medicines for women's diseases. In our previous studies, the antioxidant Nrf2 pathways were strongly induced by SWT in vitro and in vivo. Since Nrf2 activation has been associated with anticarcinogenic effects, the purpose of this study is to evaluate SWT's activity of cancer prevention. In the Ames test, SWT demonstrated an antimutagenic activity against mutagenicity induced by the chemical carcinogen 7,12-dimethylbenz(a)anthracene (DMBA). In JB6 P+ cells, a non-cancerous murine epidermal model for studying tumor promotion, SWT inhibited epidermal growth factor (EGF)-induced neoplastic transformation. The luciferase reporter gene assays demonstrated that SWT suppressed EGF-induced AP-1 and TNF-α-induced NF-κB activation, which are essential factors involved in skin carcinogenesis. In a DMBA-induced skin hyperplasia assay in 'Sensitivity to Carcinogenesis' (SENCAR) mice, both topical and oral SWT inhibited DMBA-induced epidermal hyperplasia, expression of the proliferation marker Proliferating cell nuclear antigen (PCNA), and H-ras mutations. These findings demonstrate, for the first time, that SWT prevents tumor promoter and chemical-induced carcinogenesis in vitro and in vivo, partly by inhibiting DNA damage and blocking the activation of AP-1 and NF-κB. |
| Databáze: | OpenAIRE |
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