Persistence of group A streptococci labeled with fluorescein isothiocyanate in inflammatory sites in the heart and muscle of mice and rabbits
| Autor: | M. Kaufstein, G. Arad, I. Ginsburg, Z. Zilberstein, N. Rickles, S. Kraus |
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| Rok vydání: | 1969 |
| Předmět: |
L Forms
Fluorescent Antibody Technique Group A General Biochemistry Genetics and Molecular Biology Persistence (computer science) Cell wall chemistry.chemical_compound Mice Phagocytosis Streptococcal Infections Methods Animals Fluorescein isothiocyanate Volume concentration Muscles Thigh muscle Heart Histiocytes CELL DEBRIS Fluoresceins Molecular biology Biochemistry chemistry Microscopy Fluorescence Streptolysin Rabbits Thiocyanates |
| Zdroj: | Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 131(2) |
| ISSN: | 0037-9727 |
| Popis: | SummaryA simple method of labeling living group A streptococci with fluorescein isothiocyanate (FITC) is described. Low concentrations of FITC (10-50 μg/ml) labeled group A streptococci but affected neither the viability of the cells nor the capacity of the streptococci to produce acid from glucose or to form cell-bound streptolysin S. Mucopeptide prepared from group A streptococci by the formamide method and group A streptococcal L-forms were readily labeled with FITC. On the other hand C-polysaccharide could not be labeled with FITC when in solution. Group A streptococci, streptococcal cell debris, and mucopeptide labeled with FITC injected into the thigh muscle of mice and rabbits and intramyocardially into rabbits persisted intracellularly in histiocytes for several months. Since FITC-labeled group A L-forms, which are devoid of cell walls, disappeared from the site of injection within 24-72 hr it is suggested that the long persistence of streptococci and products in the tissues is probably due to the... |
| Databáze: | OpenAIRE |
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