Identification of Differentially Expressed Genes in Sweetpotato Storage Roots Between Kokei No. 14 and Its Mutant Nongdafu 14 Using PCR-Based cDNA Subtraction
| Autor: | Yuan-jun Yang, Qingchang Liu, Shao-zhen He, Degao Liu, Hong Zhai, Wei Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Agriculture (General)
Mutant Plant Science Biology Biochemistry S1-972 Food Animals Complementary DNA suppression subtractive hybridization KEGG Carotenoid chemistry.chemical_classification Genetics differentially expressed ESTs Ecology cDNA library Wild type carotenoids food and beverages Ipomoea batatas (L.) Lam chemistry Suppression subtractive hybridization CDNA Subtraction Animal Science and Zoology Agronomy and Crop Science Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 12, Iss 4, Pp 589-595 (2013) |
| ISSN: | 2095-3119 |
| Popis: | The contents of carotenoids in the storage root of sweetpotato, Ipomoea batatas (L.) Lam. vary dramatically among different cultivars. However, so far little is known about the regulation of carotenoids synthesis in sweetpotato. In our laboratory, we identified a novel sweetpotato mutant, Nongdafu 14, which is a homogenous mutant derived from the wild type Kokei No. 14. The contents of carotenoids in the storage root of Nongdafu 14 were analyzed using high performance liquid chromatography (HPLC), and it was found that the amount of carotenoids, b-carotene, lutein and zeaxantion, three major types of carotenoids in sweetpotato storage roots, increased 2–26 folds in Nongdafu 14 compared to Kokei No. 14. Suppression subtractive hybridization (SSH) was used to identify genes that were differentially expressed in Nongdafu 14, and a differentially expressed cDNA library was constructed using the cDNA of Nongdafu 14 storage roots as tester and that of Kokei No. 14 storage roots as driver. Out of the 1 530 clones sequenced, we identified 292 nonredundant ESTs. GO and KEGG analyses of these differentially expressed ESTs indicated that diverse metabolism pathways were affected and candidate genes involved in regulation of carotenoids synthesis are suggested. |
| Databáze: | OpenAIRE |
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