Ex Vivo Adenoviral-Mediated Gene Transfer to Lung Isografts During Cold Preservation
| Autor: | Itaru Nagahiro, G. Alexander Patterson, Ralph A. Schmid, Bassem N. Mora, Teng C. Lee, Joel D. Cooper, Carlos H.R. Boasquevisque |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Genetic enhancement Genetic Vectors Pulmonary Artery medicine.disease_cause Adenoviridae Viral vector Random Allocation Gene expression medicine Animals Lung business.industry Respiratory disease Gene Transfer Techniques Organ Preservation beta-Galactosidase medicine.disease Rats Inbred F344 Rats Cold Temperature Transplantation Transplantation Isogeneic medicine.anatomical_structure Gene Expression Regulation Feasibility Studies Surgery Cardiology and Cardiovascular Medicine business Ex vivo Lung Transplantation |
| Zdroj: | The Annals of Thoracic Surgery. 63:1556-1560 |
| ISSN: | 0003-4975 |
| DOI: | 10.1016/s0003-4975(97)00237-3 |
| Popis: | Background. Although whole-organ gene transfer has been reported in heart and liver transplant models, it has not been well characterized in lung grafts. The aim of this study was to determine the feasibility of ex vivo gene transfer to rat lung isografts during cold preservation using an adenoviral vector. Methods. F344 rats, divided into four groups, underwent orthotopic left lung transplantation. In group I, lung grafts were flushed with adenovirus carrying the β-galactosidase gene. After storage at 10°C, grafts were implanted in recipient animals. Group II underwent the same procedure but graft storage was at 4°C. Groups III (10°C) and IV (4°C) served as controls. On postoperative day 5, recipients were sacrificed, and native and transplanted lungs were examined. Results. In group I, all animals showed successful, albeit patchy, gene expression. This occurred in 2 of 4 animals in group II, the other 2 showing no expression. Transduced cells were consistent morphologically with endothelial cells and pneumocytes. A minimal mononuclear inflammatory infiltrate was present. Control groups showed no transduction. Conclusions. It is feasible to perform ex vivo adenoviral-mediated gene transfer to rat lung isografts during cold preservation. (Ann Thorac Surg 1997;63:1556–61) |
| Databáze: | OpenAIRE |
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