Co-expression of tenascin and fibronectin in epithelial and stromal cells of benign lesions and ductal carcinomas in the human breast
| Autor: | Noriko Hanamura, Ilunga Kalembeyi, Akinori Ishihara, Ei-ichi Matsumoto, Teruyo Sakakura, Koji Katsuta, Toshimichi Yoshida |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Pathology
medicine.medical_specialty Stromal cell Adenocarcinoma Scirrhous Tenascin Breast Neoplasms Biology medicine.disease_cause Pathology and Forensic Medicine Papilloma Intraductal Breast Diseases Breast cancer Intraductal papilloma medicine Humans Breast RNA Messenger In Situ Hybridization Carcinoma Ductal Breast Cancer medicine.disease Fibroadenoma Immunohistochemistry Fibronectins Cancer cell biology.protein Female Carcinogenesis |
| Zdroj: | The Journal of pathology. 182(4) |
| ISSN: | 0022-3417 |
| Popis: | Tenascin (TN)-C and fibronectin (FN), which are glycoproteins of the extracellular matrix (ECM), are up-regulated in cancer tissues, including breast cancer. For assessment of their involvement in cancer invasion, it is important to know which cells are responsible for their production and secretion. The distribution of cells expressing TN and FN mRNAs in benign and malignant human breast tissues was therefore analysed by in situ hybridization, using digoxigenin-labelled cRNA probes, in addition to demonstrating the proteins immunohistochemically. Both mRNAs were expressed in epithelial cancer as well as in stromal cells in a large fraction of the tumours, with co-expression in individual cells. In cancers with intraductal components and/or those consisting of large nests, the mRNAs were more often expressed in the cancer than in the stromal cells. In scirrhous carcinomas, in contrast, the stromal cells were almost always positive for TN and FN mRNAs, while the cancer cells only rarely exhibited TN or FN expression. In benign lesions including adenosis, fibroadenoma and intraductal papilloma, the expression patterns also varied. These findings indicate that TN and FN co-expressed by cancer cells and stromal cells are probably involved in the intraductal extension and early invasion of cancer cells and in the remodelling of cancer stroma. |
| Databáze: | OpenAIRE |
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