Catalase turnover in human diploid cell cultures
| Autor: | Leonard Hayflick, W.J. Mellman, Robert T. Schimke |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Time Factors
Cell Cell Fractionation Tritium Cell Line Surface-Active Agents Methods medicine Animals Chemical Precipitation Humans Amino Acids Lung Incubation Skin Carbon Isotopes Fetus biology Immune Sera Cell Biology Fibroblasts Triazoles Catalase Diploidy Molecular biology Culture Media medicine.anatomical_structure Liver Biochemistry Cell culture biology.protein Female Rabbits Ploidy Cell fractionation |
| Zdroj: | Experimental Cell Research. 73:399-409 |
| ISSN: | 0014-4827 |
| DOI: | 10.1016/0014-4827(72)90064-x |
| Popis: | The rates of synthesis and degradation of catalase protein were measured in cultures of human diploid fibroblasts by the method of Price et al. [10] which employs 3-aminotriazole as an irreversible inhibitor of catalase without affecting its synthesis. The half-life of catalase has been estimated at approx. 30 h, a value comparable to that obtained for rat and mouse liver catalase. The turnover of catalase is influenced by a factor present in fetal calf serum which is inactivated during incubation. Suboptimal amounts of this serum factor retard both synthesis and degradation of catalase, resulting in a reduction in the cell content of catalase. From the double isotope method of Arias et al. [18] it would appear that catalase is turning over more slowly than average cellular protein. |
| Databáze: | OpenAIRE |
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