Selectin-deficiency reduces the number of spontaneous metastases in a xenograft model of human breast cancer
| Autor: | Katrin Stübke, Lena Herich, Nina Nehmann, Udo Schumacher, Daniel Wicklein |
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| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Lung Neoplasms Colorectal cancer Transplantation Heterologous Breast Neoplasms Mice Breast cancer medicine Animals Humans Mice Knockout Lung Cell adhesion molecule business.industry Cancer medicine.disease Transplantation Disease Models Animal P-Selectin medicine.anatomical_structure Oncology Bone marrow Bone Marrow Neoplasms E-Selectin business Selectin |
| Zdroj: | Cancer Letters. 321:89-99 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/j.canlet.2012.02.019 |
| Popis: | Metastasis formation is a complex process still poorly understood. Previous work in a colon cancer xenograft model showed that E(ndothelial) and P(latelet) selectins mediate spontaneous metastasis to the lungs. To investigate the functional role of selectins in breast cancer, human DU4475 breast cancer cells were injected subcutaneously into pfp-/-rag2-/- mice and in all their selectin-deficient variants (EP-/-, E-/- and P-/-). Pfp-/-rag2-/- mice as well as all their selectin-deficient variants developed primary tumours and spontaneous metastases. Compared with the wild-type mice, disseminated tumours cells were significantly lower (74% reduction, P=0.046) in the bone marrow of selectin-deficient mice. Pfp-/-rag2-/- mice developed significantly higher numbers of lung metastases (6644.83±741.77) than the E-/- (4053.33±112.58; P=0.002) and the EP-/- pfp-/-rag2-/- mice (4665.65±754.50; P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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