Muscle homeodomain protein MHox inhibits ternary complex formation at the c-fos serum response element
| Autor: | Sun-Yu Ng, Jing-Tyan Ma |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Serum Response Factor
Macromolecular Substances Response element Biophysics Muscle Proteins Biology In Vitro Techniques Biochemistry c-Fos Mice Serum response factor Animals Humans Molecular Biology Ternary complex Reporter gene Myogenesis Genes Homeobox Nuclear Proteins Cell Biology 3T3 Cells Serum Response Element Molecular biology DNA-Binding Proteins Gene Expression Regulation biology.protein Signal transduction Proto-Oncogene Proteins c-fos HeLa Cells |
| Zdroj: | Biochemical and biophysical research communications. 200(3) |
| ISSN: | 0006-291X |
| Popis: | The Serum Response Element in the c-fos promoter is the target of growth factor-regulated signal transduction pathways. The ternary complex of Serum Response Element-binding protein Serum Response Factor and its accessory protein Ternary Complex Factor are important for transcriptional stimulation of the c-fos promoter in fibroblasts. However, this promoter is repressed in differentiating muscle cells. We discovered that MHox, a muscle homeodomain protein, was capable of inhibiting formation of the ternary complex by direct physical interaction between MHox and Serum Response Factor accessory protein-1 (SAP-1), one member of the Ternary Complex Factor family of proteins. Furthermore, exogenous MHox protein inhibited serum-inducibility of a Serum Response Element-dependent reporter gene in permeabilized fibroblasts. Taken together, these results imply that MHox is involved in blocking mitogenic signals during myogenesis. |
| Databáze: | OpenAIRE |
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