Molecular cloning, organization, expression and 3D structural analysis of the MHC class Ia gene in the whitespotted bamboo shark (Chiloscyllium plagiosum)
| Autor: | Jing-Ru Wang, Meiling Lei, Xiao-Shun He, Xiu-Ming Li, Tong Shen, Jian-Ming Li |
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| Rok vydání: | 2013 |
| Předmět: |
Immunology
Molecular Sequence Data chemical and pharmacologic phenomena Sequence alignment Locus (genetics) Major histocompatibility complex Evolution Molecular Phylogenetics MHC class I Animals Amino Acid Sequence Cloning Molecular Phylogeny Genetics Polymorphism Genetic General Veterinary biology Phylogenetic tree Base Sequence Histocompatibility Antigens Class I Sequence Analysis DNA biology.organism_classification Random Amplified Polymorphic DNA Technique genomic DNA biology.protein Sharks Chiloscyllium plagiosum RNA Sequence Alignment |
| Zdroj: | Veterinary immunology and immunopathology. 157(1-2) |
| ISSN: | 1873-2534 |
| Popis: | Cartilaginous fishes are the oldest jawed vertebrates, from which the major histocompatibility complex (MHC) derived approximately 500 MYA; however, full-length genomic sequences for MHC genes in these species remain undescribed. This lack of basic information about MHC organization in cartilaginous fish is hindering investigations into the relationship between MHC polymorphism and disease, and leaves a large gap in our understanding of shark MHC evolution. Here, we obtained a complete 4887 bp genomic DNA of chplUAA (designated as chplUAA) from the whitespotted bamboo shark (Chiloscyllium plagiosum) using long PCR. The full-length cDNA sequence was 1385 bp, with a 1029 bp open reading frame (ORF) encoding 343 amino acids. Six unique sequences (chplUAA*01-06) were detected from 51 sequences from three samples. No more than two sequences were found in each individual, suggesting that only one UAA locus was amplified in each sample. Phylogenetic analysis supports monophyly of all available shark classical class Ia sequences. |
| Databáze: | OpenAIRE |
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