Persisted/recurrent hyperparathyroidism associated with development of multi-drug resistance phenotype and proliferation of parathyroid transplants
| Autor: | Maciej Lazarczyk, Małgorzata Kobylecka, Elzbieta Bogacka-Zatorska, Joanna Miskiewicz, Witold Chudziński, Magdalena Jelenska, Tomasz Grzela, Lukasz Milewski, Justyna Niderla, Piotr Dziunycz, Robert Samaha, Pawel Swiercz, Barbara Górnicka, Leszek Królicki |
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| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Pathology medicine.medical_treatment Biology Scintigraphy Parathyroid Glands In vivo Recurrence Internal medicine Genetics medicine Humans Cells Cultured Cell Proliferation Oncogene medicine.diagnostic_test Hyperparathyroidism General Medicine Middle Aged medicine.disease Molecular medicine Autotransplantation Drug Resistance Multiple Transplantation Endocrinology Phenotype Gene Expression Regulation Cell culture Parathyroid Hormone Secondary hyperparathyroidism Female Multidrug Resistance-Associated Proteins |
| Zdroj: | ResearcherID Scopus-Elsevier |
| ISSN: | 1107-3756 |
| Popis: | The surgical treatment of secondary hyperparathyroidism (HPTH) requires sub-total excision of parathyroid glands or total excision with their autotransplantation. Although this approach has been considered as a safe method of treatment, in this report we describe persisted/recurrent HPTH after parathyroid transplantation. Due to parathormone (PTH) hypersecretion and uncontrolled proliferation, the parathyroid grafts were removed and used for generation of cell cultures, which further have been subjected to in vitro studies. As a control we used parathyroid tissue, obtained during multiorgan harvesting. We found increased proliferation and up-regulated PTH production by the graft-derived, but not control in vitro cultured cells. Moreover, due to decrease of in vivo radiotracer uptake by parathyroid grafts, the expression of multi-drug resistance-involved factors, including P-glycoprotein (P-gp/mdr1), multi-drug resistance-associated protein (mrp) and bcl-2 have been investigated using RT-PCR. The analysis revealed increased expression of both, mdr1 and mrp in graft-derived cells, in contrast to control cells, which did not express P-gp/mdr1 or mrp. However, we did not observe any difference in expression of bcl-2 between analyzed cells. The up-regulated expression of P-gp/mdr1 on graft-derived cells was further confirmed by immunofluorescence studies. The described case indicates potential risk associated with transplantation of parathyroid tissue. Our results confirm a role of MDR phenomenon in occurrence of false negative results in parathyroid tissue scintigraphy studies. Moreover, they indicate that standard histological examination of transplanted material could not be sensitive enough to exclude any potential danger of abnormal graft progression. Thus, they could support the concept to use encapsulated parathyroid transplants. |
| Databáze: | OpenAIRE |
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