Inhibition of invasion and epithelial-mesenchymal transition of human breast cancer cells by hydrogen sulfide through decreased phospho-p38 expression
| Autor: | Min Lv, Ming Zhuang, Yan Li, Jin-Hai Tang, Ming-Hua Ji |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Epithelial-Mesenchymal Transition Cell Survival Glycine Breast Neoplasms Sodium hydrosulfide Biology p38 Mitogen-Activated Protein Kinases Biochemistry Transforming Growth Factor beta1 chemistry.chemical_compound Cell Movement Genetics medicine Humans Hydrogen Sulfide Viability assay Epithelial–mesenchymal transition Propidium iodide Phosphorylation Molecular Biology Cystathionine gamma-Lyase Cell cycle G1 Phase Cell Cycle Checkpoints Molecular biology Oncology chemistry Apoptosis Alkynes Cancer cell SNAI1 MCF-7 Cells Molecular Medicine Female Snail Family Transcription Factors Transcription Factors |
| Zdroj: | Molecular Medicine Reports. 10:341-346 |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2014.2161 |
| Popis: | Sodium hydrosulfide (NaHS) is an exogenous hydrogen sulfide (H2S)-releasing molecule and has antitumor potential against a wide variety of human cancer types. The effect of exogenous H2S on the invasion of breast cancer and the possible underlying mechanisms remain unknown. The present study aimed to investigate the in vitro effects of H2S on transforming growth factor-β1 (TGF-β1)-induced human breast cancer cells and the associated mechanisms. MCF-7 cells were incubated with TGF-β1 to induce epithelial-mesenchymal transition (EMT) and an MTT assay was performed to detect cell viability. Flow cytometry, using propidium iodide (PI) staining, was used to determine the stages of the cell cycle. Apoptosis was detected with Annexin V-fluorescein isothiocyanate and PI double staining. Western blotting was performed to detect the protein expression of cystathionine γ-lyase (CSE, an endogenous H2S producer), phospho-p38 (a signaling protein associated with apoptosis), and SNAI1 (Snail, associated with the induction of EMT). A Boyden chamber invasion assay was performed to detect tumor invasion. The results demonstrated that when NaHS was administered to TGF-β1-treated MCF-7 cells, the cells exhibited decreased proliferation, G0/G1 phase cell cycle arrest and increased apoptosis. NaHS treatment following TGF-β1 administration also resulted in decreased cell invasion and decreased EMT, which was indicated by decreased Snail protein expression. In addition, incubation with NaHS increased endogenous CSE protein expression and decreased p38 mitogen-activated protein kinase phosphorylation in MCF-7 cells stimulated by TGF-β1. Furthermore, the inhibition of endogenous CSE by DL-propargylglycine increased EMT in the MCF-7 cells treated with NaHS and TGF-β1. In conclusion, the present study provides insights into a novel anticancer effect of H2S on breast cancer cells through activation of the CSE/H2S pathway and decreased expression of phospho-p38. |
| Databáze: | OpenAIRE |
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