Wavelength dependence for UV-induced pyrimidine dimer formation in DNA of human peripheral blood lymphocytes
| Autor: | Steven E. Freeman, Sharon L. Ryan |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cell Survival
Ultraviolet Rays Pyrimidine dimer Toxicology Endonuclease chemistry.chemical_compound Genetics Humans Lymphocytes Molecular Biology Action spectrum Electrophoresis Agar Gel biology fungi Dose-Response Relationship Radiation DNA biology.organism_classification In vitro Wavelength Biochemistry chemistry Pyrimidine Dimers Agarose gel electrophoresis Mutation biology.protein Biophysics Micrococcus luteus |
| Zdroj: | Mutation research. 235(3) |
| ISSN: | 0027-5107 |
| Popis: | An action spectrum for the induction of pyrimidine dimers in human peripheral lymphocytes was determined between 254 and 405 nm. The presence of pyrimidine dimers was determined as UV-induced lesions that were sensitive to the dimer-specific endonuclease from Micrococcus luteus in conjunction with agarose gel electrophoresis. The rate of induction of pyrimidine dimers was maximal at 254 nm. These values can be compared with action spectra for UV-induced in vitro responses of lymphocytes. |
| Databáze: | OpenAIRE |
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