Diffusion of Protein Molecules through Microporous Nanofibrous Polyacrylonitrile Membranes
| Autor: | Yang Si, Shenghan Zhu, Gang Sun, Ameer Y. Taha, Cunyi Zhao, Kevin Bradley, Tingrui Pan |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
genetic structures
Polymers and Plastics Immobilized enzyme Diffusion Bioengineering macromolecular substances effective pore size partition coefficient Article chemistry.chemical_compound Protein purification Nanotechnology diffusion coefficient Nanofibrous membrane Micro-porous structure microporous structure Protein molecules Chemistry Process Chemistry and Technology protein-polymer interaction Organic Chemistry technology industry and agriculture Polyacrylonitrile Microporous material Membrane Chemical engineering sense organs Protein diffusion Biosensor |
| Zdroj: | ACS Appl Polym Mater ACS applied polymer materials, vol 3, iss 3 |
| Popis: | Porous nanofibrous membranes have ultrahigh specific surface areas and could be broadly employed in protein purification, enzyme immobilization, and biosensors with enhanced selectivity, sensitivity, and efficiency. However, large biomolecules, such as proteins, have hindered diffusion behavior in the micro-porous media, significantly reducing the benefits provided by the nanofibrous membranes. The study of protein diffusion in polyacrylonitrile (PAN) nanofibrous membranes produced under varied humidity and polymer concentration of electrospinning revealed that heterogeneous structures of the nanofibrous membranes possess much smaller effective pore sizes than the measured pore sizes, which significantly affects the diffusion of large molecules through the system though sizes of proteins and pH conditions also have great impacts. Only when the measured membrane pore size is at least 1000 times higher than the protein size, the diffusion behavior of the protein is predictable in the system. The results provide insights into the design and applications of proper nanofibrous materials for improved applications in protein purification and immobilizations. |
| Databáze: | OpenAIRE |
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