A highly stable, sensitive, regenerable and rapid immunoassay for detecting aflatoxin B1 in corn incorporating covalent AFB1 immobilization and a recombinant Fab antibody
| Autor: | Stephen Hearty, Om Prakash Edupuganti, Richard O'Kennedy, Soujanya Ratna Edupuganti |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Aflatoxin
Aflatoxin B1 Rapid immunoassay Recombinant fab Antibody Affinity Sensitivity and Specificity Zea mays Antibodies Analytical Chemistry Immunoglobulin Fab Fragments Antibody Specificity medicine Peptide bond media_common.cataloged_instance European Union European union media_common Immunoassay Chromatography medicine.diagnostic_test biology Chemistry Reproducibility of Results Hydrogen-Ion Concentration Recombinant Proteins Biochemistry Covalent bond biology.protein Solvents Antibody Antibodies Immobilized |
| Zdroj: | Talanta. 115 |
| ISSN: | 1873-3573 |
| Popis: | A highly robust immunoassay applicable for the detection of aflatoxin B1 (AFB1) using a Fab antibody fragment was developed. A key factor was the use of covalently immobilized AFB1 which allowed an almost three fold increase in sensitivity, reduced assay time and regeneration with retention of binding capacity. Various factors that might affect the sensitivity of the assay such as pH, organic solvents, storage stability and wash stringency were critically evaluated. It was also demonstrated that the assay was applicable for determination of AFB1 in corn samples at concentration within the European union regulatory limits. |
| Databáze: | OpenAIRE |
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