Metabolomic Analysis of Liver Tissues for Characterization of Hepatocellular Carcinoma
| Autor: | Deepak Kumar, Shisi He, Abdalla Kara Balla, Kian Ghaffari, Cristina Di Poto, Chao Tu, Alexander Kroemer, Habtom W. Ressom, Rency S. Varghese, Alessia Ferrarini, Ziling Fan, Zhenzhi Li, Meth Jayatilake, Mahlet G. Tadesse, Zaki A. Sherif |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Cirrhosis Carcinoma Hepatocellular Metabolite Biochemistry Article Gas Chromatography-Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound Metabolomics Metabolome medicine Biomarkers Tumor Humans neoplasms 030102 biochemistry & molecular biology business.industry Liver Neoplasms Lipid metabolism General Chemistry Middle Aged medicine.disease Lipid Metabolism digestive system diseases Biomarker (cell) Gene Expression Regulation Neoplastic 030104 developmental biology chemistry Liver Hepatocellular carcinoma Cancer research Female Alpha-fetoprotein business |
| Zdroj: | J Proteome Res |
| Popis: | Hepatocellular carcinoma (HCC) causes more than half a million annual deaths world-wide. Understanding the mechanisms contributing to HCC development is highly desirable for improved surveillance, diagnosis and treatment. Liver tissue metabolomics has the potential to reflect the physiological changes behind HCC development. Also, it allows identification of biomarker candidates for future evaluation in biofluids and investigation of racial disparities in HCC. Tumor and non-tumor tissues from 40 patients were analyzed by both gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) platforms to increase the metabolome coverage. The levels of the metabolites extracted from solid liver tissue of the HCC area and adjacent non-HCC area were compared. Among the analytes detected by GC-MS and LC-MS with significant alterations, 18 were selected based on biological relevance and confirmed metabolite identification. These metabolites belong to TCA cycle, glycolysis, purines, and lipid metabolism, and have been previously reported in liver metabolomic studies where high correlation with HCC progression is implied. We demonstrated that metabolites related to HCC pathogenesis can be identified through liver tissue metabolomic analysis. Additionally, this study has enabled us to identify race-specific metabolites associated with HCC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|