Matrix metalloproteinases MMP-2, -9 and tissue inhibitors TIMP-1, -2 expression and secretion by primary human osteoblast cells in response to titanium, zirconia, and alumina ceramics

Autor: Dominique Laurent-Maquin, Yannick Josset, Chantal Trenteseaux, Roselyne Garnotel, Zohra Oum'Hamed
Přispěvatelé: Interfaces biomatériaux/tissus hôtes, Université de Reims Champagne-Ardenne (URCA)-IFR53-Institut National de la Santé et de la Recherche Médicale (INSERM), UFR Médecine-1 (UFR), IFR 53, Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Médicaments : Dynamique Intracellulaire et Architecture Nucléaire (MéDIAN), Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Laurent-Maquin, Dominique
Jazyk: angličtina
Rok vydání: 2004
Předmět:
MESH: DNA Primers
Proteases
Ceramics
Materials science
Biomedical Engineering
Context (language use)
Enzyme-Linked Immunosorbent Assay
Matrix metalloproteinase
MESH: Base Sequence
MESH: Gelatinase B
MESH: Gelatinase A
Osseointegration
Bone remodeling
Biomaterials
Extracellular matrix
03 medical and health sciences
0302 clinical medicine
MESH: Ceramics
MESH: Aluminum Oxide
MESH: Reverse Transcriptase Polymerase Chain Reaction
Bone cell
medicine
Aluminum Oxide
Humans
030304 developmental biology
DNA Primers
Titanium
[SDV.IB] Life Sciences [q-bio]/Bioengineering
0303 health sciences
Tissue Inhibitor of Metalloproteinase-2
MESH: Osteoblasts
Osteoblasts
Tissue Inhibitor of Metalloproteinase-1
MESH: Humans
Base Sequence
Reverse Transcriptase Polymerase Chain Reaction
MESH: Tissue Inhibitor of Metallo
MESH: Enzyme-Linked Immunosorbent Assay
Osteoblast
030206 dentistry
Cell biology
medicine.anatomical_structure
Matrix Metalloproteinase 9
MESH: Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase 2
[SDV.IB]Life Sciences [q-bio]/Bioengineering
Zirconium
Biomedical engineering
Zdroj: Journal of Biomedical Materials Research Part A
Journal of Biomedical Materials Research Part A, 2004, 68, pp.114-22. ⟨10.1002/jbm.a.20001⟩
Journal of Biomedical Materials Research Part A, Wiley, 2004, 68, pp.114-22. ⟨10.1002/jbm.a.20001⟩
ISSN: 1549-3296
1552-4965
DOI: 10.1002/jbm.a.20001⟩
Popis: Osteogenic properties of bone cells are a key parameter governing osseointegration of implant devices. In this context, osteoblasts have a central role via extracellular matrix synthesis and remodeling that they regulate through different protease activity. In this study, we have analyzed the expression of two matrix metalloproteinases (MMPs): MMP-2 (72 kDa) and MMP-9 (92 kDa) and their specific tissue inhibitors TIMP-1 and TIMP-2 in primary human osteoblastic cells. The effect of titanium, zirconia, and alumina ceramics on the synthesis of these proteases was assessed using reverse transcriptase-polymerase chain reaction, enzyme-linked immunosorbent assay, and zymographic analysis. Our results showed that osteoblasts express MMP-2 and -9 mRNA. Furthermore, MMP-2 mRNA expression was decreased by titanium and increased by alumina whereas zirconia did not have any significant effect. Conversely, MMP-9 mRNA expression was stimulated by titanium but decreased with zirconia, whereas alumina induced no significant changes. Zymographic analysis has evidenced pro-MMP-2 gelatinolytic activity in all cell populations with time-dependent increase profile; pro-MMP-9, however, was not detected. Enzyme-linked immunosorbent assay data confirmed the production of MMP-2 and very low levels of MMP-9. In addition, TIMP-1 was secreted in 24-h-cultured cells and increased to maximal level at 48-72 h whereas TIMP-2 levels were very low. The interactions between human osteoblasts and the studied biomaterials altered both MMP-2, -9 and TIMP-1expression indicating that biomaterials may influence osseointegration and bone remodeling.
Databáze: OpenAIRE
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